Following Ribosome Footprints to Understand Translation at a Genome Wide Level

Protein translation is a key step in gene expression. The development of Ribosome Profiling has allowed the global analysis of this process at sub-codon resolution. In the last years the method has been applied to several models ranging from bacteria to mammalian cells yielding a surprising amount o...

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Bibliographic Details
Published in:Computational and structural biotechnology journal 2018-01, Vol.16, p.167-176
Main Authors: Eastman, Guillermo, Smircich, Pablo, Sotelo-Silveira, José R.
Format: Article
Language:English
Subjects:
Review
Ribo-seq
Ribosome profiling
Transcriptome
Translation
Translatome
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Summary:Protein translation is a key step in gene expression. The development of Ribosome Profiling has allowed the global analysis of this process at sub-codon resolution. In the last years the method has been applied to several models ranging from bacteria to mammalian cells yielding a surprising amount of insight on the mechanism and the regulation of translation. In this review we describe the key aspects of the experimental protocol and comment on the main conclusions raised in different models.
ISSN:2001-0370
2001-0370
DOI:10.1016/j.csbj.2018.04.001