A Perspective on Extracellular Vesicles Proteomics

Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to ide...

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Bibliographic Details
Published in:Frontiers in chemistry 2017-11, Vol.5, p.102-102
Main Authors: Rosa-Fernandes, Livia, Rocha, Victória Bombarda, Carregari, Victor Corasolla, Urbani, Andrea, Palmisano, Giuseppe
Format: Article
Language:English
Subjects:
bottom-up
Chemistry
exosomes
extracellular vesicles
mass spectrometry
post-translational modification
proteomics
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Summary:Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2017.00102