Analysis of Thiodiphenol in Rat Urine as a Biomarker of Exposure to Temephos

Temephos is an organophosphorus pesticide widely used as a larvicide in public health campaigns to control vector-borne diseases. Data on the urinary elimination of temephos metabolites are limited, and there is no validated biomarker of exposure for its evaluation. This study aimed to determine the...

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Bibliographic Details
Published in:Journal of xenobiotics 2024-12, Vol.14 (4), p.1889-1900
Main Authors: Shih, Miao-Ling, López-González, Ma de Lourdes, Uribe-Ramírez, Marisela, Rojas-García, Aurora Elizabeth, Verdín-Betancourt, Francisco Alberto, Sierra-Santoyo, Adolfo
Format: Article
Language:English
Subjects:
Biomarkers
BPS
Dengue fever
Dosimetry
Laboratory animals
Metabolites
Nitrogen
organophosphorus pesticides
Pesticides
Risk assessment
Sodium
temephos
thiodiphenol
Toxicity
Urine
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Summary:Temephos is an organophosphorus pesticide widely used as a larvicide in public health campaigns to control vector-borne diseases. Data on the urinary elimination of temephos metabolites are limited, and there is no validated biomarker of exposure for its evaluation. This study aimed to determine the urinary excretion kinetics of temephos and its metabolites in adult male rats. Hence, adult male Wistar rats were administered orally with a single dose of temephos (300 mg/kg). Urine samples were collected at different time intervals after dosing and enzymatically hydrolyzed using β-glucuronidase/sulfatase from . The metabolites were extracted and analyzed by HPLC-DAD. The metabolites detected were 4,4'-thiodiphenol (TDP), 4,4'-sulfinyldiphenol (SIDP), 4,4'-sulfonyldiphenol (SODP), or bisphenol S (BPS), a non-identified metabolite, and only traces of the parent compound. The mean urine concentrations of metabolites were used for kinetic analysis. Urinary levels of TDP were fitted to a two-compartmental model, and its half-lives ( ) were 27.8 and 272.1 h for the first and second phases, respectively. The of BPS was 17.7 h. TDP, the main metabolite of temephos, was eliminated by urine and is specific and stable. Therefore, it may be used as a biomarker of temephos exposure.
ISSN:2039-4713
2039-4705
2039-4713
DOI:10.3390/jox14040100