New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap

exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a with worldwide intere...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-01, Vol.10 (1), p.69
Main Authors: Chambard, Marie, Plasson, Carole, Derambure, Céline, Coutant, Sophie, Tournier, Isabelle, Lefranc, Benjamin, Leprince, Jérôme, Kiefer-Meyer, Marie-Christine, Driouich, Azeddine, Follet-Gueye, Marie-Laure, Boulogne, Isabelle
Format: Article
Language:English
Subjects:
Biochemistry
Biochemistry, Molecular Biology
Biofilms
Biotechnology
Cell Behavior
Cellular Biology
Chromosomes
Chromosomes, Plant - genetics
Deoxyribonucleic acid
Development Biology
DNA
DNA, Plant - metabolism
Extracellular Space - metabolism
Extracellular Traps - metabolism
Genetic testing
Genomes
Genomics
Glycine max
Glycine max (L.) Merr
Glycine max - metabolism
high-throughput DNA sequencing
Life Sciences
Mitochondria
Mitochondrial DNA
Molecular biology
Neutrophils
Nucleotide sequence
Organelles
Organelles - metabolism
PEP-13
Peptides
Phytopathology and phytopharmacy
Plant breeding
plant exDNA
Plant Roots - metabolism
root extracellular trap (RET)
Soybeans
Subcellular Processes
Vegetal Biology
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Summary:exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a with worldwide interest: soybean ( (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus ). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10010069