QTL Analysis of Resistance to High-Intensity UV-B Irradiation in Soybean ( Glycine max [L.] Merr.)

• Published in: International journal of molecular sciences 2019-07, Vol.20 (13), p.3287
• Main Authors: Yoon, Min Young, Kim, Moon Young, Ha, Jungmin, Lee, Taeyoung, Kim, Kyung Do, Lee, Suk-Ha
• Format: Article
• Language: English
• Subjects: bZIP transcription factor21/TGA9; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Crosses, Genetic; Gene expression; Gene Expression Regulation, Plant; Gene mapping; Genes; Genes, Plant; Genetic Linkage; Genome, Plant; Genotypes; Glycine max - genetics; Glycine max - radiation effects; Immune response; Inbreeding; leucine-rich repeat family protein; Lod Score; Mapping; Morphology; Mutation; Ozonosphere; Phenotype; Physiology; Plant Leaves - radiation effects; Polymorphism, Single Nucleotide - genetics; Population; Proteins; Quantitative trait loci; Quantitative Trait Loci - genetics; Radiation Tolerance - genetics; Sequence analysis; Single-nucleotide polymorphism; soybean; Soybeans; spectrin beta chain, brain; stress defense signaling; Stress, Physiological - genetics; Stress, Physiological - radiation effects; Synteny - genetics; Ultraviolet radiation; Ultraviolet Rays; UV-B stress resistance
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20133287

High-intensity ultraviolet-B (UV-B) irradiation is a complex abiotic stressor resulting in excessive light exposure, heat, and dehydration, thereby affecting crop yields. In the present study, we identified quantitative trait loci (QTLs) for resistance to high-intensity UV-B irradiation in soybean ( [L.]). We used a genotyping-by-sequencing approach using an F6 recombinant inbred line (RIL) population derived from a cross between Cheongja 3 (UV-B sensitive) and Buseok (UV-B resistant). We evaluated the degree of leaf damage by high-intensity UV-B radiation in the RIL population and identified four QTLs, , , , and , for UV-B stress resistance, together explaining 20% of the observed phenotypic variation. The genomic regions containing and and their syntenic blocks included other known biotic and abiotic stress-related QTLs. The QTL with the highest logarithm of odds (LOD) score of 3.76 was on Chromosome 12, containing two genes encoding spectrin beta chain, brain (SPTBN, Glyma.12g088600) and bZIP transcription factor21/TGACG motif-binding 9 (bZIP TF21/TGA9, Glyma.12g088700). Their amino acid sequences did not differ between the mapping parents, but both genes were significantly upregulated by UV-B stress in Buseok but not in Cheongja 3. Among five genes in on Chromosome 6, Glyma.06g319700 (encoding a leucine-rich repeat family protein) had two nonsynonymous single nucleotide polymorphisms differentiating the parental lines. Our findings offer powerful genetic resources for efficient and precise breeding programs aimed at developing resistant soybean cultivars to multiple stresses. Furthermore, functional validation of the candidate genes will improve our understanding of UV-B stress defense mechanisms.