Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome

Stress is one of the major causes of irritable bowel syndrome (IBS), which is well-known for perturbing the microbiome and exacerbating IBS-associated symptoms. However, changes in the gut microbiome and metabolome in response to colorectal distention (CRD), combined with restraint stress (RS) admin...

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Bibliographic Details
Published in:Scientific reports 2021-09, Vol.11 (1), p.17596-17596, Article 17596
Main Authors: Hu, Yue, Chen, Fang, Ye, Haiyong, Lu, Bin
Format: Article
Language:English
Subjects:
631/326/2565/2134
692/4020/1503/1581/2071
Animals
Biosynthesis
Digestive system
Dysbacteriosis
Gastrointestinal Microbiome
Humanities and Social Sciences
Intestinal microflora
Intestine
Irritable bowel syndrome
Irritable Bowel Syndrome - etiology
Irritable Bowel Syndrome - metabolism
Irritable Bowel Syndrome - microbiology
Irritable Bowel Syndrome - pathology
Male
Metabolic pathways
Metabolism
Metabolites
Metabolome
Microbiomes
Microbiota
multidisciplinary
Phenotypes
Phenylalanine
Rats
Rats, Sprague-Dawley
Rodents
rRNA 16S
Science
Science (multidisciplinary)
Stress
Stress, Psychological - complications
Tryptophan
Tyrosine
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Summary:Stress is one of the major causes of irritable bowel syndrome (IBS), which is well-known for perturbing the microbiome and exacerbating IBS-associated symptoms. However, changes in the gut microbiome and metabolome in response to colorectal distention (CRD), combined with restraint stress (RS) administration, remains unclear. In this study, CRD and RS stress were used to construct an IBS rat model. The 16S rRNA gene sequencing was used to characterize the microbiota in ileocecal contents. UHPLC-QTOF-MS/MS assay was used to characterize the metabolome of gut microbiota. As a result, s ignificant gut microbial dysbiosis was observed in stress-induced IBS rats, with the obvious enrichment of three and depletion of 11 bacterial taxa in IBS rats, when compared with those in the control group (q 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-97083-z