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Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

A segregating population with 410 F2 individuals from the cross MERCIA (Rht-B1a) × Dwarf 123 was made to identify a new major dwarfing gene carrying by novel wheat germplasm Dwarf 123. Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms amo...

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Published in:	Journal of Integrative Agriculture 2013-05, Vol.12 (5), p.749-755
Main Authors:	MENG, Ya-ning, KANG, Su-hua, LAN, Su-que, LI, Xing-pu, ZHANG, Ye-lun, BAI, Feng
Format:	Article
Language:	English
Subjects:	chromosome chromosome dwarfing gene genetic distance linkage analysis molecular markers polymorphism Triticum aestivum L dwarfing gene F2群体 genetic distance linkage analysis molecular markers polymorphism SSR标记 Triticum aestivum L 基因连锁分析挂钩普通小麦标记鉴定矮秆基因遗传距离
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creator	MENG, Ya-ning KANG, Su-hua LAN, Su-que LI, Xing-pu ZHANG, Ye-lun BAI, Feng
description	A segregating population with 410 F2 individuals from the cross MERCIA (Rht-B1a) × Dwarf 123 was made to identify a new major dwarfing gene carrying by novel wheat germplasm Dwarf 123. Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.
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Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.</description><identifier>ISSN: 2095-3119</identifier><identifier>EISSN: 2352-3425</identifier><identifier>DOI: 10.1016/S2095-3119(13)60266-X</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>chromosome ; chromosome dwarfing gene genetic distance linkage analysis molecular markers polymorphism Triticum aestivum L ; dwarfing gene ; F2群体 ; genetic distance ; linkage analysis ; molecular markers ; polymorphism ; SSR标记 ; Triticum aestivum L ; 基因连锁分析 ; 挂钩 ; 普通小麦 ; 标记鉴定 ; 矮秆基因 ; 遗传距离</subject><ispartof>Journal of Integrative Agriculture, 2013-05, Vol.12 (5), p.749-755</ispartof><rights>2013 Chinese Academy of Agricultural Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. 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Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.</description><subject>chromosome</subject><subject>chromosome dwarfing gene genetic distance linkage analysis molecular markers polymorphism Triticum aestivum L</subject><subject>dwarfing gene</subject><subject>F2群体</subject><subject>genetic distance</subject><subject>linkage analysis</subject><subject>molecular markers</subject><subject>polymorphism</subject><subject>SSR标记</subject><subject>Triticum aestivum L</subject><subject>基因连锁分析</subject><subject>挂钩</subject><subject>普通小麦</subject><subject>标记鉴定</subject><subject>矮秆基因</subject><subject>遗传距离</subject><issn>2095-3119</issn><issn>2352-3425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqFUU1v1DAUjCoqtSr9CZXMjR4Cz3ZsJyeEFigrLUViQfRmOfZz6t2uTZ1AKb8ed1N65WT7fcyMZ6rqjMIrClS-XjPoRM0p7V5Sfi6BSVlfHVTHjAtW84aJZ-X-b-SoOh3HDQBQIQBke1ytlg7jFHywZgopkuTJev2FfDJ5i5msQtyiI1MippQ2KZN3dyb7EAdygRFJiGSRdruy9_0azfS8OvTmZsTTx_Ok-vbh_dfFx3r1-WK5eLuqbSPYVCsluGx9g1wq0XmmwJaHMsYKiy2zyvkWXdHIOuWcsALbXlGUivXU9Wj5SbWccV0yG_0jh53J9zqZoPeFlAdt8hTsDWonaWHBxiJiA2g71TjRY4fYM9Y2pmCdz1h3JnoTB71JP3Ms6vWfId5vf2tkQDkUu1iZFfOszWkcM_onbgr6IQ29T0M_WK0p1_s09FXZO5v3vEnaDDmMenFZYCUH6ECU_pu5j8W0XwGzHm3AaNGFjHYqvwr_ZXjxqOw6xeG2BPQkrRFSNkIB_wuexqRA</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>MENG, Ya-ning</creator><creator>KANG, Su-hua</creator><creator>LAN, Su-que</creator><creator>LI, Xing-pu</creator><creator>ZHANG, Ye-lun</creator><creator>BAI, Feng</creator><general>Elsevier B.V</general><general>Science Press</general><general>Institute of Cereal and 0il Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Research Station of Crop Gene Resource&Germplasm Enhancement, Ministry of Agriculture/Key Laboratory of Genetics and Breeding of Hebei Province, Shijiazhuang 050031, P.R.China%College of Life Science, Hebei Normal University, Shijiazhuang 050016, P.R.China</general><general>Elsevier</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>DOA</scope></search><sort><creationdate>201305</creationdate><title>Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat</title><author>MENG, Ya-ning ; 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Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. 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subjects	chromosome chromosome dwarfing gene genetic distance linkage analysis molecular markers polymorphism Triticum aestivum L dwarfing gene F2群体 genetic distance linkage analysis molecular markers polymorphism SSR标记 Triticum aestivum L 基因连锁分析挂钩普通小麦标记鉴定矮秆基因遗传距离
title	Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat
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