Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum

Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-08, Vol.25 (17), p.9467
Main Authors: Yan, Xiuxiu, Wu, Xiaojuan, Sun, Fengcheng, Nie, Hushuai, Du, Xiaohong, Li, Xiaolei, Fang, Yongyu, Zhai, Yongqing, Zhao, Yan, Fan, Bobo, Ma, Yanhong
Format: Article
Language:English
Subjects:
Abiotic stress
Agricultural production
Agropyron - genetics
Agropyron - metabolism
Agropyron mongolicum
AmGDSL1 gene
antioxidant activity
Antioxidants
Cloning
Cloning, Molecular
Cold
Cytoplasm
Drought
drought resistance
Droughts
Gene expression
Gene Expression Regulation, Plant
Genomes
Leaves
Lipase - genetics
Lipase - metabolism
Localization
MicroRNAs
Nicotiana - genetics
Nicotiana - metabolism
Physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
Plants, Genetically Modified - genetics
Seeds
Stress response
Stress, Physiological - genetics
Tobacco
Wheat
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Summary:Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a gene from , designated as , was successfully cloned and isolated. The subcellular localization of the gene (pCAMBIA1302- -EGFP) results showed that the AmGDSL1 protein of was only localized in the cytoplasm. When transferred into tobacco ( ), the heterologous expression of led to enhanced drought tolerance. Under drought stress, overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in for enhancing crop drought resistance.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25179467