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Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum
Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a...
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| Published in: | International journal of molecular sciences 2024-08, Vol.25 (17), p.9467 |
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| container_issue | 17 |
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| container_title | International journal of molecular sciences |
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| creator | Yan, Xiuxiu Wu, Xiaojuan Sun, Fengcheng Nie, Hushuai Du, Xiaohong Li, Xiaolei Fang, Yongyu Zhai, Yongqing Zhao, Yan Fan, Bobo Ma, Yanhong |
| description | Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a
gene from
, designated as
, was successfully cloned and isolated. The subcellular localization of the
gene (pCAMBIA1302-
-EGFP) results showed that the AmGDSL1 protein of
was only localized in the cytoplasm. When transferred into tobacco (
), the heterologous expression of
led to enhanced drought tolerance. Under drought stress,
overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the
gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in
for enhancing crop drought resistance. |
| doi_str_mv | 10.3390/ijms25179467 |
| format | article |
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gene from
, designated as
, was successfully cloned and isolated. The subcellular localization of the
gene (pCAMBIA1302-
-EGFP) results showed that the AmGDSL1 protein of
was only localized in the cytoplasm. When transferred into tobacco (
), the heterologous expression of
led to enhanced drought tolerance. Under drought stress,
overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the
gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in
for enhancing crop drought resistance.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25179467</identifier><identifier>PMID: 39273413</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abiotic stress ; Agricultural production ; Agropyron - genetics ; Agropyron - metabolism ; Agropyron mongolicum ; AmGDSL1 gene ; antioxidant activity ; Antioxidants ; Cloning ; Cloning, Molecular ; Cold ; Cytoplasm ; Drought ; drought resistance ; Droughts ; Gene expression ; Gene Expression Regulation, Plant ; Genomes ; Leaves ; Lipase - genetics ; Lipase - metabolism ; Localization ; MicroRNAs ; Nicotiana - genetics ; Nicotiana - metabolism ; Physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - genetics ; Plant Roots - metabolism ; Plants, Genetically Modified - genetics ; Seeds ; Stress response ; Stress, Physiological - genetics ; Tobacco ; Wheat</subject><ispartof>International journal of molecular sciences, 2024-08, Vol.25 (17), p.9467</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c310t-15b8a52a74cd804d736fa71297d605acc1b400d5c7de3c9da93fdcb5dec52ae3</cites><orcidid>0000-0002-0581-4404 ; 0009-0007-0770-0113</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3104099521/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3104099521?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39273413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Xiuxiu</creatorcontrib><creatorcontrib>Wu, Xiaojuan</creatorcontrib><creatorcontrib>Sun, Fengcheng</creatorcontrib><creatorcontrib>Nie, Hushuai</creatorcontrib><creatorcontrib>Du, Xiaohong</creatorcontrib><creatorcontrib>Li, Xiaolei</creatorcontrib><creatorcontrib>Fang, Yongyu</creatorcontrib><creatorcontrib>Zhai, Yongqing</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Fan, Bobo</creatorcontrib><creatorcontrib>Ma, Yanhong</creatorcontrib><title>Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a
gene from
, designated as
, was successfully cloned and isolated. The subcellular localization of the
gene (pCAMBIA1302-
-EGFP) results showed that the AmGDSL1 protein of
was only localized in the cytoplasm. When transferred into tobacco (
), the heterologous expression of
led to enhanced drought tolerance. Under drought stress,
overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the
gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in
for enhancing crop drought resistance.</description><subject>Abiotic stress</subject><subject>Agricultural production</subject><subject>Agropyron - genetics</subject><subject>Agropyron - metabolism</subject><subject>Agropyron mongolicum</subject><subject>AmGDSL1 gene</subject><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Cold</subject><subject>Cytoplasm</subject><subject>Drought</subject><subject>drought resistance</subject><subject>Droughts</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genomes</subject><subject>Leaves</subject><subject>Lipase - genetics</subject><subject>Lipase - metabolism</subject><subject>Localization</subject><subject>MicroRNAs</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Seeds</subject><subject>Stress response</subject><subject>Stress, Physiological - genetics</subject><subject>Tobacco</subject><subject>Wheat</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkctLAzEQh4MoPqo3z7LgxYPVPDfJSUp9FQoe6j1kk-ySspvUZPfQ_97VVhFPMwzffAzzA-ASwTtCJLz36y5jhrikJT8Ap4hiPIWw5Id_-hNwlvMaQkwwk8fghEjMCUXkFDzM2xh8aAodbPE8BNP7GHRbrPrBbotYF7Pu5XG1RIUPxaxJcbNNMRRdDE1svRm6c3BU6za7i32dgPfnp_f563T59rKYz5ZTQxDsp4hVQjOsOTVWQGo5KWvNEZbclpBpY1BFIbTMcOuIkVZLUltTMevMuOXIBCx2Whv1Wm2S73Taqqi9-h7E1Cidem9ap2zJBUSOG01rKgwWgjKBJa5LgYSFcnTd7FybFD8Gl3vV-Wxc2-rg4pDVeDBlRHIOR_T6H7qOQxr_s6OglAyjkbrdUSbFnJOrfw9EUH1lpP5mNOJXe-lQdc7-wj-hkE_dO4pJ</recordid><startdate>20240830</startdate><enddate>20240830</enddate><creator>Yan, Xiuxiu</creator><creator>Wu, Xiaojuan</creator><creator>Sun, Fengcheng</creator><creator>Nie, Hushuai</creator><creator>Du, Xiaohong</creator><creator>Li, Xiaolei</creator><creator>Fang, Yongyu</creator><creator>Zhai, Yongqing</creator><creator>Zhao, Yan</creator><creator>Fan, Bobo</creator><creator>Ma, Yanhong</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0581-4404</orcidid><orcidid>https://orcid.org/0009-0007-0770-0113</orcidid></search><sort><creationdate>20240830</creationdate><title>Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum</title><author>Yan, Xiuxiu ; Wu, Xiaojuan ; Sun, Fengcheng ; Nie, Hushuai ; Du, Xiaohong ; Li, Xiaolei ; Fang, Yongyu ; Zhai, Yongqing ; Zhao, Yan ; Fan, Bobo ; Ma, Yanhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-15b8a52a74cd804d736fa71297d605acc1b400d5c7de3c9da93fdcb5dec52ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abiotic stress</topic><topic>Agricultural production</topic><topic>Agropyron - genetics</topic><topic>Agropyron - metabolism</topic><topic>Agropyron mongolicum</topic><topic>AmGDSL1 gene</topic><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Cold</topic><topic>Cytoplasm</topic><topic>Drought</topic><topic>drought resistance</topic><topic>Droughts</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genomes</topic><topic>Leaves</topic><topic>Lipase - genetics</topic><topic>Lipase - metabolism</topic><topic>Localization</topic><topic>MicroRNAs</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Physiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Seeds</topic><topic>Stress response</topic><topic>Stress, Physiological - genetics</topic><topic>Tobacco</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Xiuxiu</creatorcontrib><creatorcontrib>Wu, Xiaojuan</creatorcontrib><creatorcontrib>Sun, Fengcheng</creatorcontrib><creatorcontrib>Nie, Hushuai</creatorcontrib><creatorcontrib>Du, Xiaohong</creatorcontrib><creatorcontrib>Li, Xiaolei</creatorcontrib><creatorcontrib>Fang, Yongyu</creatorcontrib><creatorcontrib>Zhai, Yongqing</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Fan, Bobo</creatorcontrib><creatorcontrib>Ma, Yanhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest - 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It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a
gene from
, designated as
, was successfully cloned and isolated. The subcellular localization of the
gene (pCAMBIA1302-
-EGFP) results showed that the AmGDSL1 protein of
was only localized in the cytoplasm. When transferred into tobacco (
), the heterologous expression of
led to enhanced drought tolerance. Under drought stress,
overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the
gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in
for enhancing crop drought resistance.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39273413</pmid><doi>10.3390/ijms25179467</doi><orcidid>https://orcid.org/0000-0002-0581-4404</orcidid><orcidid>https://orcid.org/0009-0007-0770-0113</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; ProQuest - Publicly Available Content Database |
| subjects | Abiotic stress Agricultural production Agropyron - genetics Agropyron - metabolism Agropyron mongolicum AmGDSL1 gene antioxidant activity Antioxidants Cloning Cloning, Molecular Cold Cytoplasm Drought drought resistance Droughts Gene expression Gene Expression Regulation, Plant Genomes Leaves Lipase - genetics Lipase - metabolism Localization MicroRNAs Nicotiana - genetics Nicotiana - metabolism Physiology Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - genetics Plant Roots - metabolism Plants, Genetically Modified - genetics Seeds Stress response Stress, Physiological - genetics Tobacco Wheat |
| title | Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum |
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