IgG Anti-IgE Autoantibodies in Visceral Leishmaniasis

• Published in: Memórias do Instituto Oswaldo Cruz 2002-01, Vol.97 (1), p.101-103
• Main Authors: Atta, AM, Sousa-Atta, MLB, D'Oliveira, A, Almeida, RP, Araújo, MI, Carvalho, EM
• Format: Article
• Language: English
• Subjects: Antibodies, Anti-Idiotypic - blood; Antibodies, Anti-Idiotypic - immunology; Autoantibodies - blood; Autoantibodies - immunology; GTP-Binding Proteins - metabolism; Humans; IgE; IgE, IgG anti-IgE, protein G, visceral leishmaniasis, schistosomiasis mansoni; IgG anti-IgE; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Leishmania; Leishmaniasis, Visceral - blood; Leishmaniasis, Visceral - immunology; PARASITOLOGY; Protein Binding; protein G; schistosomiasis mansoni; Schistosomiasis mansoni - blood; Schistosomiasis mansoni - immunology; Sepharose - metabolism; TROPICAL MEDICINE; visceral leishmaniasis
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762002000100017

Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.