The Effects of Cholestasis and Cirrhosis on Gastric Acid and Pepsin Secretions in Rat: Involvement of Nitric Oxide

Objective(s)The liver has major role in the organism homeostasis, interactions with other systems, synthesis and metabolism of bile production, drug detoxification and hormone inactivation. Cholestasis can be defined as an impairment of the bile flow which can lead to hepatocytes necrosis and finall...

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Bibliographic Details
Published in:Iranian journal of basic medical sciences 2010-09, Vol.13 (47)
Main Authors: Fatemeh Nabavizadeh, Rohallah Moloudi, Ahmad Reza Dehpour, Hossein Nahrevanian, Kaveh Shahvaisi, Ehsan Salimi
Format: Article
Language:English
Subjects:
Cholestasis
Gastric acid
Liver cirrhosis
Nitric oxide
Pepsin
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Summary:Objective(s)The liver has major role in the organism homeostasis, interactions with other systems, synthesis and metabolism of bile production, drug detoxification and hormone inactivation. Cholestasis can be defined as an impairment of the bile flow which can lead to hepatocytes necrosis and finally cirrhosis. Some studies reported a gastric acid secretion reduction in cirrhotic subjects, while others reported normal production gastric acid secretion. Our aim was to evaluate the effects of cholestasis and cirrhosis on gastric acid and pepsin secretions and its possible mechanism in rat.Materials and MethodsMale Wistar rats were randomly divided into five groups (n= 8): control, cholestasis, sham cholestasis, cirrhosis and sham cirrhosis. Laparatomy was done under general anesthesia and then bile duct ligation (BDL) was performed. After 2 and 4 weeks in cholestasis and cirrhosis groups respectively, gastric content was collected by wash-out technique. Basal and stimulated acid and pepsin secretions were measured by using titration and the Anson method respectively in all groups. In order to measure stimulated acid and pepsin secretions, pentagastrin (25 µg/kg, i.p.) was used. Nitric Oxide (NO) metabolites of gastric tissue were determined by Griess microassy method.ResultsAcid and pepsin secretions were significantly reduced in cholestatic and cirrhotic rats in comparison with control and sham groups (P< 0.01). NO metabolite of gastric tissue was significantly increased in cholestatic and cirrhotic rats (P< 0.01).ConclusionReducing of gastric acid and pepsin output in cholestatic and cirrhotic rats may be due to increasing in NO content of gastric tissue.
ISSN:2008-3866
2008-3874