Molecular Analysis of MgO Nanoparticle-Induced Immunity against Fusarium Wilt in Tomato

Fusarium wilt, caused by f. sp. (FOL), is a devastating soilborne disease in tomatoes. Magnesium oxide nanoparticles (MgO NPs) induce strong immunity against Fusarium wilt in tomatoes. However, the mechanisms underlying this immunity remain poorly understood. Comparative transcriptome analysis and m...

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Published in:International journal of molecular sciences 2023-02, Vol.24 (3), p.2941
Main Authors: Takehara, Yushi, Fijikawa, Isamu, Watanabe, Akihiro, Yonemura, Ayumi, Kosaka, Tomoyuki, Sakane, Kosei, Imada, Kiyoshi, Sasaki, Kazunori, Kajihara, Hiroshi, Sakai, Shoji, Mizukami, Yoichi, Haider, Muhammad Salman, Jogaiah, Sudisha, Ito, Shin-Ichi
Format: Article
Language:English
Subjects:
Cell walls
Disease control
Encyclopedias
Environmental impact
Fungicides
Fusarium
Fusarium - genetics
Fusarium oxysporum
Genes
Genomes
Glycine
glycine-rich protein
Immunity
Infections
Inoculation
Magnesium
Magnesium Oxide
NAD(P)H oxidase
Nanoparticles
Pest control
Pesticides
plant defense
Plant diseases
Plant Diseases - genetics
Roots
Soil-borne diseases
Solanum lycopersicum
Solanum lycopersicum - genetics
Superconductors (materials)
Tomatoes
transcriptome
Transcriptomes
Wilt
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Summary:Fusarium wilt, caused by f. sp. (FOL), is a devastating soilborne disease in tomatoes. Magnesium oxide nanoparticles (MgO NPs) induce strong immunity against Fusarium wilt in tomatoes. However, the mechanisms underlying this immunity remain poorly understood. Comparative transcriptome analysis and microscopy of tomato roots were performed to determine the mechanism of MgO NP-induced immunity against FOL. Eight transcriptomes were prepared from tomato roots treated under eight different conditions. Differentially expressed genes were compared among the transcriptomes. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that in tomato roots pretreated with MgO NPs, encoding apoplastic protease and encoding NADPH oxidase were upregulated when challenge-inoculated with FOL. The gene encoding glycine-rich protein 4 (SlGRP4) was chosen for further analysis. was rapidly transcribed in roots pretreated with MgO NPs and inoculated with FOL. Immunomicroscopy analysis showed that SlGRP4 accumulated in the cell walls of epidermal and vascular vessel cells of roots pretreated with MgO NPs, but upon FOL inoculation, SlGRP4 further accumulated in the cell walls of cortical tissues within 48 h. The results provide new insights into the probable mechanisms of MgO NP-induced tomato immunity against Fusarium wilt.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032941