Oleanolic acid: A promising antidiabetic metabolite detected in Aglianico grape pomace

• Published in: Journal of functional foods 2023-05, Vol.104, p.105548, Article 105548
• Main Authors: Errichiello, Francesco, D'Amato, Maria, Gambuti, Angelita, Moio, Luigi, Pastore, Arianna, AL-Hmadi, Hekmat, Stornaiuolo, Mariano, Serino, Elena, Taglialatela-Scafati, Orazio, Forino, Martino
• Format: Article
• Language: English
• Subjects: Antidiabetic metabolite; Grape pomace valorization; Natural triterpenoid; Oleanolic acid; Red grapes
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2023.105548

[Display omitted] •Red grape pomace was chemically characterized by NMR, LC-MS and HPLC.•Oleanolic acid is the major bioactive metabolite recoverable from red grape pomace.•Oleanolic acid influenced the glucose uptake activity of C2C12 myoblast.•Oleanolic acid influenced the mitochondrial activity of C2C12 myoblast. Grape pomace, a bulky component of winery waste, is a source of healthy compounds. So far, scientific research has mainly focused on its polyphenol content, but given the impressive number of bioactivities shown by grape pomace, it is not unlikely that, besides polyphenols, additional metabolites, so far undetected, may be involved. In order to verify such hypothesis, an in-depth chemical analysis of Aglianico (Vitis vinifera) grape pomace was conducted by NMR and LC-MS/MS. In addition to a number of polyphenols, a remarkable concentration of oleanolic acid (0.45 mg/g - fresh weight) was determined in the analyzed material. Oleanolic acid is a natural triterpenoid showing many bioactivities including antitumor, anti-inflammatory, antibiotic and antiviral properties. Also, it was proven a potential antidiabetic molecule in Type1 Diabetes rats. Hence, its influence on the mitochondrial and glucose uptake activities of C2C12 myoblast was here assessed, thus supporting oleanolic acid as a promising antidiabetic metabolite.