Transfusion of Polynitroxylated Pegylated Hemoglobin Stabilizes Pial Arterial Dilation and Decreases Infarct Volume After Transient Middle Cerebral Artery Occlusion

• Published in: Journal of the American Heart Association 2017-09, Vol.6 (9), p.n/a
• Main Authors: Cao, Suyi, Zhang, Jian, Ma, Li, Hsia, Carleton J. C., Koehler, Raymond C.
• Format: Article
• Language: English
• Subjects: Animals; Cerebral Arteries - drug effects; Cerebral Arteries - physiopathology; cerebral blood flow; Cerebrovascular Circulation - drug effects; Collateral Circulation - drug effects; hemoglobin; Hemoglobins - administration & dosage; Infarction, Middle Cerebral Artery - drug therapy; Infarction, Middle Cerebral Artery - pathology; Infarction, Middle Cerebral Artery - physiopathology; Infusions, Intravenous; ischemic stroke; Male; Neuroprotective Agents - administration & dosage; Original Research; Pia Mater - blood supply; pial vessels; rats; Rats, Wistar; Reperfusion Injury - pathology; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Time Factors; Vasodilation - drug effects
• ISSN: 2047-9980; 2047-9980
• DOI: 10.1161/JAHA.117.006505

Background Polynitroxylation of hemoglobin confers superoxide dismutase–mimetic and peroxidase activity and may protect from reperfusion injury in addition to facilitating oxygen transport. We determined whether transfusion of polynitroxylated PEGylated hemoglobin (PNPH) is protective in the rat filament model of 2 hours of middle cerebral artery occlusion (MCAO). Methods and Results Transfusion of 10 mL/kg of PNPH at 20 minutes of MCAO reduced infarct volume by over 70% (n=10). To determine whether PNPH might act by promoting vasodilation, pial arteriolar diameter in the distal MCA border region was measured in closed cranial windows. With no transfusion, MCAO induced an initial dilation (36±2% ±SE) that subsided by 2 hours (5±4%; n=8). With PNPH transfusion at 20 minutes of MCAO, the initial dilation (31±3%) was better maintained at 2 hours (21±4%; n=7; P