Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system

The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene edi...

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Bibliographic Details
Published in:Stem cell research 2022-05, Vol.61, p.102764-102764
Main Authors: Liu, Yu, Jin, Yinge, Chen, Tao, Wu, Yunqin, Peng, Xiaohua, Li, Wansheng, Wei, Shu, Chen, Min, Zou, Qingjian, Guo, Suqin, Xu, Jucai, Tang, Chengcheng, Zhou, Xiaoqing
Format: Article
Language:English
Subjects:
Cell Line
CRISPR-Cas Systems - genetics
Gene Editing
GTPase-Activating Proteins
Homozygote
Humans
Induced Pluripotent Stem Cells
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Summary:The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102764