Ilimaquinone Induces Apoptosis and Autophagy in Human Oral Squamous Cell Carcinoma Cells

In this study, the anti-tumor activity of ilimaquinone (IQ), a sesquiterpene quinone isolated from marine sponge Halichondria sp., in oral squamous cell carcinoma (OSCC) cells, was investigated. IQ suppressed the viability of the OSCC cell lines SCC4 and SCC2095 with IC50 values of 7.5 and 8.5 μM, r...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2020-08, Vol.8 (9), p.296
Main Authors: Lin, Cheng-Wen, Bai, Li-Yuan, Su, Jui-Hsin, Chiu, Chang-Fang, Lin, Wei-Yu, Huang, Wei-Ting, Shih, Ming-Cheng, Huang, Yu-Ting, Hu, Jing-Lan, Weng, Jing-Ru
Format: Article
Language:English
Subjects:
AKT protein
Antibodies
Antioxidants
Antitumor agents
Apoptosis
Autophagy
Cancer therapies
Caspase
Chloroquine
Colorectal cancer
Flow cytometry
ilimaquinone
Intelligence
Mcl-1 protein
NMR
Nuclear magnetic resonance
Oral squamous cell carcinoma
OSCC
p53
p53 Protein
Phagocytosis
Proteins
Quinones
Reactive oxygen species
Sesquiterpene quinone
Squamous cell carcinoma
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, the anti-tumor activity of ilimaquinone (IQ), a sesquiterpene quinone isolated from marine sponge Halichondria sp., in oral squamous cell carcinoma (OSCC) cells, was investigated. IQ suppressed the viability of the OSCC cell lines SCC4 and SCC2095 with IC50 values of 7.5 and 8.5 μM, respectively. Flow cytometric analysis demonstrated that IQ induced caspase-dependent apoptosis in SCC4 cells and modulated the expression of several cell growth-related gene products, including Akt, p38, Mcl-1, and p53. Notably, p53 knockdown caused higher resistance to IQ’s anti-tumor activity. In addition, IQ increased reactive oxygen species generation, which was partially reversed by the addition of antioxidants. Furthermore, it triggered autophagy, as evidenced by acidic organelle formation and LC3B-II and Atg5 expression in SCC4 cells. Pretreatment with the autophagy inhibitor 3-methyladenine or chloroquine partially decreased IQ-induced apoptosis, suggesting that IQ induced protective autophagy. In summary, IQ has potential to be used in OSCC therapy.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines8090296