Mangifera indica leaves extract and mangiferin modulate CB1 and PPARγ receptors and others markers associated with obesity

• Published in: Journal of functional foods 2019-05, Vol.56, p.74-83
• Main Authors: Brito, Larissa Froede, Gontijo, Douglas Costa, Toledo, Renata Celi Lopes, Barcelos, Rafael Mazioli, de Oliveira, Alaíde Braga, Brandão, Geraldo Célio, de Sousa, Lirlândia Pires, Ribeiro, Sônia Machado Rocha, Leite, João Paulo Viana, Fietto, Luciano Gomes, de Queiroz, José Humberto
• Format: Article
• Language: English
• Subjects: Adipogenesis; Adiponectin; CB1 and PPARγ receptors; Leptin; Mangifera indica; Mangiferin; Obesity; Resistin
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.03.003

[Display omitted] •Phytochemical characterization of ethanolic extract of the leaves from Mangifera indica.•Analyzes with extract of M. indica and its isolated majority compound (mangiferin).•Cytotoxic and anti-inflammatory evaluation of M. indica and mangiferin in vitro.•Post and co-treatment in vivo evaluated with M. indica and mangiferin.•Activity differences between extracts of M. indica and mangiferin. This study aimed phytochemical characterization (UPLC-DAD-MS/MS) of ethanolic extract of the leaves from Mangifera indica (EMI) and Mangiferin (MAN), analysis of the cytotoxic (MTT) and anti-inflammatory potential (expression of TNF-α) of EMI and MAN in vitro. In addition, was evaluate the effect on the mRNA expression of genes (CB1, PPARγ, adiponectin, resistin and leptin) associated with adipogenesis in adipose tissue of rats fed a cafeteria diet. Thus, wistar rats were treated by gavage with EMI and MAN for several days (according to the post and co-treatments). The adipose tissue was weighed and checked the expression of different markers by RT-PCR. The presence of MAN as major compound in EMI was verified. Both EMI and MAN were not cytotoxic, with lower EMI expression of TNF-α. Furthermore, EMI and MAN had proadipogenic action on post-treatment, while in the co-treatment, EMI attenuated the effect of adipogenesis and MAN increased the adipogenic process.