Elucidating the potential of cellulases isolated from a locally isolated strain (NSF-2) in paper and pulp industry

• Published in: Cleaner Materials 2022-12, Vol.6, p.100139, Article 100139
• Main Authors: Devi, Anita, Jaryal, Rajneesh, Bhatia, Chahat, Singh, Namita
• Format: Article
• Language: English
• Subjects: Cellulases; De-inking; Enzyme Purification; Industrial Green Technology; Recycling of Waste Paper
• ISSN: 2772-3976; 2772-3976
• DOI: 10.1016/j.clema.2022.100139

[Display omitted] •For the purpose of human consumption of enzymes, the potential of enzyme cellulases which are known to all has made it pave its way in the field of present-day biotechnology and bio-based emerging industries specifically the paper and pulp industries which generates a daily waste of tremendous amount.•The current study deals with the characterization and applicability of cellulases fabricated by a locally isolated strain (NSF-2) and the purification and characterization of the enzyme. Purified enzyme showed the best activity at pH 5, 50 °C and 3 % substrate concentration.•Salts like SDS, urea, EDTA are perceived to have a regressive effect on enzyme activity while tween20 and tween100 are neutral towards enzyme. Hg2+ is found to be the most potent inhibitor and Ca2+ is found to be the most significant activator of the enzyme respectively.•Enzyme is found to be highly suitable for application in deinking industries as the inked paper when treated with the purified enzyme shows appreciable results during observations done by conducting innovative research and methods to detect the quality of deinked paper.•The patent has been achieved for the innovative method applied to detect the quality of the paper treated with enzyme which can be used in paper and pulp industry. The potential of enzyme cellulases which are known to all has made it pave its way in the field of present-day biotechnology and bio-based emerging industries. For the purpose of human consumption of enzymes, it makes it even more demanding to perform highly precised purification method. Our study deals with the characterization and applicability of cellulases fabricated by a locally isolated strain (NSF-2). The enzyme was purified using standard purification techniques. The isolated enzyme was observed to be of molecular weight 75KDa Purified enzyme showed the best activity at pH5, 50 °C and 3 % substrate concentration. The gene isolated from cellulase enzyme of about 1.2 Kb was amplified from selected isolate. Salts like SDS, urea, EDTA were perceived to have a regressive effect on enzyme activity while tween 20 and tween 100 were neutral towards enzyme. Hg2+ was found to be the most potent inhibitor and Ca2+ was found to be the most potent inhibitor and most significant activator of the enzyme respectively. The waste paper was deinked with isolated enzyme, sodium silicate (5 %) and NaOH (2 %). Various techniques like FTIR, SEM, fluorescence micrographs and fluorescence spec