Expression of the SIRT2 gene and its relationship with body size traits in Qinchuan cattle (Bos taurus)

Silent information regulator 2 (SIRT2) is a member of the sirtuin family of class III NAD (nicotinamide adenine dinucleotide)-dependent protein deacetylases and may regulate senescence, metabolism and apoptosis. The aims of this study were to investigate whether the SIRT2 gene could be used as a can...

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Bibliographic Details
Published in:International journal of molecular sciences 2015-01, Vol.16 (2), p.2458-2471
Main Authors: Gui, Lin-Sheng, Zhang, Ya-Ran, Liu, Gui-Yao, Zan, Lin-Sen
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Alleles
Animals
Body Size
body size traits
Bos taurus
Cattle
Cloning, Molecular
expression study
Gene expression
Gene Frequency
Genotype
Haplotypes
Linkage Disequilibrium
Phenotype
Phylogeny
Polymerase chain reaction
Polymorphism, Single Nucleotide
Proteins
Qinchuan cattle
RNA, Messenger - metabolism
Sequence Analysis, DNA
SIRT2 gene
Sirtuin 2 - classification
Sirtuin 2 - genetics
Sirtuin 2 - metabolism
Studies
Subcutaneous Fat - metabolism
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Summary:Silent information regulator 2 (SIRT2) is a member of the sirtuin family of class III NAD (nicotinamide adenine dinucleotide)-dependent protein deacetylases and may regulate senescence, metabolism and apoptosis. The aims of this study were to investigate whether the SIRT2 gene could be used as a candidate gene in the breeding of Qinchuan cattle. Real-time polymerase chain reaction (RT-PCR) results showed that among all types of tissue that were analyzed, the highest mRNA expression levels of the gene were found in subcutaneous fat. DNA sequencing of 468 individual Qinchuan cattle identified two novel, single nucleotide polymorphisms (g.19501 C > T and g.19518 C > T) in the 3' untranslated region (3'UTR) of the SIRT2 gene. The frequencies of SNP g.19501 C > T and g.19518 C > T were in Hardy-Weinberg disequilibrium in all the samples (chi-square test, χ2 < χ0.052). An association analysis showed that the two loci were significantly correlated with some body size traits and the H2H2 (-CT-CT-) diplotypes performed better than other combinations. These results indicated that the variations in the SIRT2 gene and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms16022458