Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis

• Published in: Parasite (Paris) 2024, Vol.31, p.65
• Main Authors: Cheng, Yong Kang, Zhang, Yao, Zhang, Zhao Yu, Cong, Pei Kun, Feng, Ji Yu, Zhang, Ru, Long, Shao Rong, Zhang, Xi, Wang, Zhong Quan, Cui, Jing
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Cloning, Molecular; Female; glutamate dehydrogenase; Glutamate Dehydrogenase - genetics; Glutamate Dehydrogenase - metabolism; Helminth Proteins - genetics; Helminth Proteins - metabolism; Larva - enzymology; Larva - genetics; Larva - growth & development; Male; metabolism; molting; Phylogeny; RNA Interference; Sequence Alignment; trichinella spiralis; Trichinella spiralis - enzymology; Trichinella spiralis - genetics; Trichinella spiralis - growth & development
• ISSN: 1776-1042; 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2024065

Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs.