Palmitoylation Is Indispensable for Remorin to Restrict Tobacco Mosaic Virus Cell-to-Cell Movement in Nicotiana benthamiana

Remorin (REM) is a plant-specific plasma membrane-associated protein regulating plasmodesmata plasticity and restricting viral cell-to-cell movement. Here, we show that palmitoylation is broadly present in group 1 remorin proteins in and is crucial for plasma membrane localization and accumulation....

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Bibliographic Details
Published in:Viruses 2022-06, Vol.14 (6), p.1324
Main Authors: Ma, Tingting, Fu, Shuai, Wang, Kun, Wang, Yaqin, Wu, Jianxiang, Zhou, Xueping
Format: Article
Language:English
Subjects:
Cell Movement
Cloning
Lipoylation
Localization
Maximum likelihood method
Membrane proteins
movement
Movement protein
Nicotiana - metabolism
Nicotiana benthamiana
Palmitoylation
Permeability
Phylogenetics
Plant Diseases
Plant Proteins
Plant Viral Movement Proteins - genetics
Plant Viral Movement Proteins - metabolism
Plasma
plasma membrane
Plasmodesmata
Proteins
remorin
tobacco mosaic virus
Tobacco Mosaic Virus - physiology
Viruses
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Summary:Remorin (REM) is a plant-specific plasma membrane-associated protein regulating plasmodesmata plasticity and restricting viral cell-to-cell movement. Here, we show that palmitoylation is broadly present in group 1 remorin proteins in and is crucial for plasma membrane localization and accumulation. By screening the four members of group 1 remorin proteins, we found that only NbREM1.5 could significantly hamper tobacco mosaic virus (TMV) cell-to-cell movement. We further showed that NbREM1.5 interacts with the movement protein of TMV in vivo and interferes with its function of expanding the plasmodesmata size exclusion limit. We also demonstrated that palmitoylation is indispensable for NbREM1.5 to hamper plasmodesmata permeability and inhibit TMV cell-to-cell movement.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14061324