Microsatellite marker-assisted backcross breeding for improvement of wheat salt tolerance using Kharchia 65

Salinity is a significant abiotic stress that affects plants from germination through all growth stages. This study was aimed to determine the morpho-physiological and genetic variations in BC F , BC F and F generations resulting from the cross combination WH1105 × Kharchia 65. A significant reducti...

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Bibliographic Details
Published in:BMC genomics 2024-06, Vol.25 (1), p.550-7
Main Authors: Sharma, Kritika, Yashveer, Shikha, Singh, Vikram, Sharma, Sudhir, Redhu, Mandeep, Rahimi, Mehdi
Format: Article
Language:English
Subjects:
Abiotic stress
Agricultural production
Analysis
Cell division
Cluster analysis
Crosses, Genetic
Diseases and pests
Genes
Genetic aspects
Genetic diversity
Genetic markers
Genetic relationship
Genetic testing
Genotype
Germination
Germination - genetics
Grain
Growth
Heritability
Marker-assisted selection
Methods
Microsatellite instability
Microsatellite Repeats - genetics
Microsatellites
Morphology
Nax1 and Nax2
Phenotype
Physiology
Plant breeding
Plant Breeding - methods
Polymorphism
Population genetics
Principal components analysis
Salinity
Salinity tolerance
Salt
Salt tolerance
Salt Tolerance - genetics
Seeds
Sodium
SSR markers
Triticum - genetics
Triticum - growth & development
Wheat
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Summary:Salinity is a significant abiotic stress that affects plants from germination through all growth stages. This study was aimed to determine the morpho-physiological and genetic variations in BC F , BC F and F generations resulting from the cross combination WH1105 × Kharchia 65. A significant reduction in germination percentage was observed under salt stress in BC F and F seeds. Correlation, heritability in the broad sense, phenotypic coefficient of variability (PCV) and genotypic coefficient of variability (GCV) were measured for all traits. The presence of both Nax1 and Nax2 loci was confirmed in twenty-nine plants using the marker-assisted selection technique. Genetic relationships among the populations were assessed using twenty-four polymorphic SSR markers. Cluster analysis along with two and three-dimensional PCA scaling (Principal Component Analysis) revealed the distinct nature of WH 1105 and Kharchia 65. Six plants closer to the recurrent parent (WH1105) selected through this study can serve as valuable genetic material for salt-tolerant wheat improvement programs.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-024-10468-y