Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection

A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized , '-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functio...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2018-04, Vol.18 (4), p.1173
Main Authors: Ariffin, Eda Yuhana, Tan, Ling Ling, Abd Karim, Nurul Huda, Yook Heng, Lee
Format: Article
Language:English
Subjects:
Aminopropyltriethoxysilane
Biosensors
Dengue fever
dengue virus detection
Deoxyribonucleic acid
DNA
Imines
Nanospheres
nickel(II) salphen complex
optical DNA biosensor
Phenylenediamine
Piperidine
porous silica nanospheres
Reflectance
reflectance measurement
Response time
synthetic DNA binder
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Summary:A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized , '-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 × 10 -1.0 × 10 M (R² = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes.
ISSN:1424-8220
1424-8220
DOI:10.3390/s18041173