Quantitative Determination of Steroidal Saponins of Semen Allii Fistulosi Using HPLC-ELSD and HPLC-MS

In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of . The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.9...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2024, Vol.2024 (1), p.1872918
Main Authors: Zheng, Zhancai, Xu, Deduo, Lu, Wenquan, Chen, Wansheng, Yang, Yingbo, Wu, Zhijun
Format: Article
Language:English
Subjects:
Analysis
Antioxidants
Cancer
Chinese medicine
Chromatography
Drugs
High performance liquid chromatography
Ischemia
Mass spectrometry
Methods
Quantitative analysis
Reproducibility
Saponins
Scientific imaging
Semen
Water
Citations: Items that this one cites
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Summary:In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of . The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.91%∼99.77%. Similarly, the HPLC-MS method was rapid, sensitive, accurate, and reproducible, and the recoveries ranging from 95.61% to 99.70% in the control samples. The sensitivity of the HPLC-MS method was several times higher than that of the HPLC-ELSD method. These results collectively demonstrated that these methods are suitable for the content determination of steroidal saponins of and the identification of its plant source.
ISSN:2090-8865
2090-8873
DOI:10.1155/2024/1872918