Prevention of di (2-ethylhexyl) Phthalate-induced Testicular Disturbance in Mice by Co-administration of L-carnitine

di (2-ethylhexyl) phthalate (DEHP) is widely used in the plastic industry and can induce reproductive toxicity. On the other hand, L-carnitine (LC) plays a crucial role in sperm metabolism and maturation. This study evaluates the effect of LC on body and testis weight, testis tissue, count, motility...

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Bibliographic Details
Published in:International journal of fertility & sterility 2011-10, Vol.5 (3), p.186-192
Main Authors: Zare, Zohre, Mohammadi, Moslem, Eimani, Hossein, Shafaroudi, Majid Malekzadeh
Format: Article
Language:English
Subjects:
carnitine
ethylhexyl) phthalate
Original
spermatogenesis
testis
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Summary:di (2-ethylhexyl) phthalate (DEHP) is widely used in the plastic industry and can induce reproductive toxicity. On the other hand, L-carnitine (LC) plays a crucial role in sperm metabolism and maturation. This study evaluates the effect of LC on body and testis weight, testis tissue, count, motility, viability, morphology, and chromatin quality of epididymal sperm, testicular spermatid number (TSN) per gram testis and daily sperm production (DSP) in LC-treated mice. IN THIS EXPERIMENTAL STUDY, ADULT MALE NMRI MICE (MEAN AGE: 4 weeks) were given doses of DEHP and LC by gavaging for 2 weeks. All samples were assessed according to World Health Organization (WHO) criteria. Sperm morphology was assessed using Papanicolaou staining and sperm chromatin quality by aniline-blue staining. The left testes were fixed in Bouinś solution for histological examination and the end slices were stained with hematoxylin and eosin (H&E). The right testes were homogenized, and then TSN and DSP were calculated with an improved Neubauer haemocytometer and respective frames. Paired t-test, ANOVA, and Kruskal-Wallis tests were utilized for data analysis. Co-administration of DEHP and LC not only prevented significant gains in testicular weight, but also maintained the sperm's normal morphology and chromatin quality (p
ISSN:2008-076X
2008-0778