Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which ca...

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Bibliographic Details
Published in:Communications biology 2023-09, Vol.6 (1), p.992-992, Article 992
Main Authors: Monfort, Tual, Azzollini, Salvatore, Brogard, Jérémy, Clémençon, Marilou, Slembrouck-Brec, Amélie, Forster, Valerie, Picaud, Serge, Goureau, Olivier, Reichman, Sacha, Thouvenin, Olivier, Grieve, Kate
Format: Article
Language:English
Subjects:
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631/1647/245/2226
631/1647/328/1650
631/532/2064/2158
631/61/2320
Biology
Biomedical and Life Sciences
Cell culture
Cell size
Data acquisition
Explants
Life Sciences
Microscopes
Mitosis
Organelles
Organoids
Pluripotency
Retina
Rosette formation
Tomography
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Summary:Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which can be coupled to a commercial microscope with a stage top incubator, allowing non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. Long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids is demonstrated, highlighting tissue and cell organization processes such as rosette formation and mitosis as well as cell shape and motility. Imaging on retinal explants highlights single 3D cone and rod structures. An optimal workflow for data acquisition, postprocessing and saving is demonstrated, resulting in a time gain factor of 10 compared to prior state of the art. Finally, a method to increase D-FFOCT signal-to-noise ratio is demonstrated, allowing rapid organoid screening. Dynamic full-field optical coherence tomography coupled to a commercial microscope with a stage top incubator, allows non-invasive, long term, label-free longitudinal imaging of biological specimens.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-023-05378-w