Sequestration of RBM10 in Nuclear Bodies: Targeting Sequences and Biological Significance

• Published in: International journal of molecular sciences 2021-09, Vol.22 (19), p.10526
• Main Authors: Wang, Ling-Yu, Xiao, Sheng-Jun, Kunimoto, Hiroyuki, Tokunaga, Kazuaki, Kojima, Hirotada, Kimura, Masatsugu, Yamamoto, Takahiro, Yamamoto, Naoki, Zhao, Hong, Nishio, Koji, Tani, Tokio, Nakajima, Koichi, Sunami, Kishiko, Inoue, Akira
• Format: Article
• Language: English
• Subjects: Alternative splicing; C2H2 Zn finger; Deletion mutant; Localization; Mutation; nuclear body-targeting sequence; Nuclei (cytology); Nucleoli; Proteins; RBM10; RNA-binding protein; S1-1 nuclear body; Splicing; Transcription
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms221910526

RBM10 is an RNA-binding protein that regulates alternative splicing (AS). It localizes to the extra-nucleolar nucleoplasm and S1-1 nuclear bodies (NBs) in the nucleus. We investigated the biological significance of this localization in relation to its molecular function. Our analyses, employing deletion mutants, revealed that RBM10 possesses two S1-1 NB-targeting sequences (NBTSs), one in the KEKE motif region and another in the C2H2 Zn finger (ZnF). These NBTSs act synergistically to localize RBM10 to S1-1 NBs. The C2H2 ZnF not only acts as an NBTS, but is also essential for AS regulation by RBM10. Moreover, RBM10 does not participate in S1-1 NB formation, and without alterations of RBM10 protein levels, its NB-localization changes, increasing as cellular transcriptional activity declines, and vice versa. These results indicate that RBM10 is a transient component of S1-1 NBs and is sequestered in NBs via its NBTSs when cellular transcription decreases. We propose that the C2H2 ZnF exerts its NB-targeting activity when RBM10 is unbound by pre-mRNAs, and that NB-localization of RBM10 is a mechanism to control its AS activity in the nucleus.