Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum in...

Full description

Saved in:
Bibliographic Details
Published in:Horticultural science (Praha) 2022-01, Vol.49 (1), p.48-51
Main Authors: Poniewozik, Monika, Parzymies, Marzena, Szot, Paweł
Format: Article
Language:English
Subjects:
Activated carbon
Activated charcoal
Ascorbic acid
Charcoal
lady slipper
Leaves
Micropropagation
Morphogenesis
Multiplication
orchid
Paphiopedilum
Phenolic compounds
Phenols
Rooting
Shoots
tissue culture
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13
cites cdi_FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13
container_end_page 51
container_issue 1
container_start_page 48
container_title Horticultural science (Praha)
container_volume 49
creator Poniewozik, Monika
Parzymies, Marzena
Szot, Paweł
description Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.
doi_str_mv 10.17221/68/2020-HORTSCI
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_d7db70d7f59542aa803beb3a4a2f142b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_d7db70d7f59542aa803beb3a4a2f142b</doaj_id><sourcerecordid>2644765841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13</originalsourceid><addsrcrecordid>eNo9UctqHDEQHEICXmzffRTkPLHe0hzD4sQLBgc_IDfReu1omR1NpFmTvfnTsw-TU9PV1VUF1TQ3BH8jilJyK_UtxRS3949PL8_L1admQTQWbccY-9wssJa01VL9vmiua91gjInouCZ40bzfxRjcjHJE4Ob0BnPwyPVQXIYBwegRVJeLTe5wTx7lEaURvaW5ZLTNZerzOoyhpnri5tanfu9L_ruf-jDmoSKXxzmM8_HrF0x9ylPwadhtD0BN6zFcNV8iDDVcf8zL5vXH3cvyvn14_Llafn9oHediboEKoZ2ITksWVBDYKms58wqc8vq4OKtiAOkxwcBAyg6Ywgq6jgYRCLtsVmddn2FjppK2UPYmQzInIJe1gTInNwTjlbcKexVFJzgF0JjZYBlwoJFwag9aX89aU8l_dqHOZpN3ZTzEN1RyrqTQ_OiIzyxXcq0lxP-uBJtTbUZqc6zNfNTG_gGHvY4X</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2644765841</pqid></control><display><type>article</type><title>Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne</title><source>Publicly Available Content Database</source><creator>Poniewozik, Monika ; Parzymies, Marzena ; Szot, Paweł</creator><creatorcontrib>Poniewozik, Monika ; Parzymies, Marzena ; Szot, Paweł</creatorcontrib><description>Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.</description><identifier>ISSN: 0862-867X</identifier><identifier>EISSN: 1805-9333</identifier><identifier>DOI: 10.17221/68/2020-HORTSCI</identifier><language>eng</language><publisher>Prague: Czech Academy of Agricultural Sciences (CAAS)</publisher><subject>Activated carbon ; Activated charcoal ; Ascorbic acid ; Charcoal ; lady slipper ; Leaves ; Micropropagation ; Morphogenesis ; Multiplication ; orchid ; Paphiopedilum ; Phenolic compounds ; Phenols ; Rooting ; Shoots ; tissue culture</subject><ispartof>Horticultural science (Praha), 2022-01, Vol.49 (1), p.48-51</ispartof><rights>2022. This work is published under https://www.agriculturejournals.cz/web/about/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13</citedby><cites>FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13</cites><orcidid>0000-0002-4835-3804</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2644765841?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590</link.rule.ids></links><search><creatorcontrib>Poniewozik, Monika</creatorcontrib><creatorcontrib>Parzymies, Marzena</creatorcontrib><creatorcontrib>Szot, Paweł</creatorcontrib><title>Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne</title><title>Horticultural science (Praha)</title><description>Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.</description><subject>Activated carbon</subject><subject>Activated charcoal</subject><subject>Ascorbic acid</subject><subject>Charcoal</subject><subject>lady slipper</subject><subject>Leaves</subject><subject>Micropropagation</subject><subject>Morphogenesis</subject><subject>Multiplication</subject><subject>orchid</subject><subject>Paphiopedilum</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Rooting</subject><subject>Shoots</subject><subject>tissue culture</subject><issn>0862-867X</issn><issn>1805-9333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNo9UctqHDEQHEICXmzffRTkPLHe0hzD4sQLBgc_IDfReu1omR1NpFmTvfnTsw-TU9PV1VUF1TQ3BH8jilJyK_UtxRS3949PL8_L1admQTQWbccY-9wssJa01VL9vmiua91gjInouCZ40bzfxRjcjHJE4Ob0BnPwyPVQXIYBwegRVJeLTe5wTx7lEaURvaW5ZLTNZerzOoyhpnri5tanfu9L_ruf-jDmoSKXxzmM8_HrF0x9ylPwadhtD0BN6zFcNV8iDDVcf8zL5vXH3cvyvn14_Llafn9oHediboEKoZ2ITksWVBDYKms58wqc8vq4OKtiAOkxwcBAyg6Ywgq6jgYRCLtsVmddn2FjppK2UPYmQzInIJe1gTInNwTjlbcKexVFJzgF0JjZYBlwoJFwag9aX89aU8l_dqHOZpN3ZTzEN1RyrqTQ_OiIzyxXcq0lxP-uBJtTbUZqc6zNfNTG_gGHvY4X</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Poniewozik, Monika</creator><creator>Parzymies, Marzena</creator><creator>Szot, Paweł</creator><general>Czech Academy of Agricultural Sciences (CAAS)</general><general>Czech Academy of Agricultural Sciences</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4835-3804</orcidid></search><sort><creationdate>20220101</creationdate><title>Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne</title><author>Poniewozik, Monika ; Parzymies, Marzena ; Szot, Paweł</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Activated carbon</topic><topic>Activated charcoal</topic><topic>Ascorbic acid</topic><topic>Charcoal</topic><topic>lady slipper</topic><topic>Leaves</topic><topic>Micropropagation</topic><topic>Morphogenesis</topic><topic>Multiplication</topic><topic>orchid</topic><topic>Paphiopedilum</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Rooting</topic><topic>Shoots</topic><topic>tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poniewozik, Monika</creatorcontrib><creatorcontrib>Parzymies, Marzena</creatorcontrib><creatorcontrib>Szot, Paweł</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Agriculture Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Horticultural science (Praha)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poniewozik, Monika</au><au>Parzymies, Marzena</au><au>Szot, Paweł</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne</atitle><jtitle>Horticultural science (Praha)</jtitle><date>2022-01-01</date><risdate>2022</risdate><volume>49</volume><issue>1</issue><spage>48</spage><epage>51</epage><pages>48-51</pages><issn>0862-867X</issn><eissn>1805-9333</eissn><abstract>Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.</abstract><cop>Prague</cop><pub>Czech Academy of Agricultural Sciences (CAAS)</pub><doi>10.17221/68/2020-HORTSCI</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-4835-3804</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0862-867X
ispartof Horticultural science (Praha), 2022-01, Vol.49 (1), p.48-51
issn 0862-867X
1805-9333
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_d7db70d7f59542aa803beb3a4a2f142b
source Publicly Available Content Database
subjects Activated carbon
Activated charcoal
Ascorbic acid
Charcoal
lady slipper
Leaves
Micropropagation
Morphogenesis
Multiplication
orchid
Paphiopedilum
Phenolic compounds
Phenols
Rooting
Shoots
tissue culture
title Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T21%3A55%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20activated%20charcoal%20and%20ascorbic%20acid%20on%20in%20vitro%20morphogenesis%20and%20o-dihydroxyphenols%20content%20in%20Paphiopedilum%20insigne&rft.jtitle=Horticultural%20science%20(Praha)&rft.au=Poniewozik,%20Monika&rft.date=2022-01-01&rft.volume=49&rft.issue=1&rft.spage=48&rft.epage=51&rft.pages=48-51&rft.issn=0862-867X&rft.eissn=1805-9333&rft_id=info:doi/10.17221/68/2020-HORTSCI&rft_dat=%3Cproquest_doaj_%3E2644765841%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c445t-a2558c5fc863e7e50b7bb43d7ac7d8b7bbcb7fea6d010a3a669a3707a992e5e13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2644765841&rft_id=info:pmid/&rfr_iscdi=true