Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque

Microendoscopic calcium imaging with one-photon miniature microscopes enables unprecedented readout of neural circuit dynamics during active behavior in rodents. In this study, we describe successful application of this technology in the rhesus macaque, demonstrating plug-and-play, head-mounted reco...

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Bibliographic Details
Published in:Cell reports (Cambridge) 2021-06, Vol.35 (11), p.109239-109239, Article 109239
Main Authors: Bollimunta, Anil, Santacruz, Samantha R, Eaton, Ryan W, Xu, Pei S, Morrison, John H, Moxon, Karen A, Carmena, Jose M, Nassi, Jonathan J
Format: Article
Language:English
Subjects:
Animals
Behavior, Animal - physiology
Calcium - metabolism
calcium imaging
Endoscopy
GCaMP
GRIN lens
Head
Imaging, Three-Dimensional
Macaca mulatta
macaque
Male
microendoscopy
miniscope
Motor Cortex - diagnostic imaging
Motor Cortex - surgery
Neurons - physiology
Time Factors
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Summary:Microendoscopic calcium imaging with one-photon miniature microscopes enables unprecedented readout of neural circuit dynamics during active behavior in rodents. In this study, we describe successful application of this technology in the rhesus macaque, demonstrating plug-and-play, head-mounted recordings of cellular-resolution calcium dynamics from large populations of neurons simultaneously in bilateral dorsal premotor cortices during performance of a naturalistic motor reach task. Imaging is stable over several months, allowing us to longitudinally track individual neurons and monitor their relationship to motor behavior over time. We observe neuronal calcium dynamics selective for reach direction, which we could use to decode the animal's trial-by-trial motor behavior. This work establishes head-mounted microendoscopic calcium imaging in macaques as a powerful approach for studying the neural circuit mechanisms underlying complex and clinically relevant behaviors, and it promises to greatly advance our understanding of human brain function, as well as its dysfunction in neurological disease.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2021.109239