Effects of Irradiation on Biology and Mating Behaviour of Wild Males of Brown Marmorated Stink Bug Using a 6 MV Medical Linear Accelerator

• Published in: Insects (Basel, Switzerland) Switzerland), 2023-05, Vol.14 (5), p.460
• Main Authors: Roselli, Gerardo, Anfora, Gianfranco, Suckling, David Maxwell, Mazzoni, Valerio, Vanoni, Valentina, Menegotti, Loris, Fellin, Lorenzo, Rossi Stacconi, Marco Valerio, Ioriatti, Claudio, Cristofaro, Massimo
• Format: Article
• Language: English
• Subjects: Adaptability; Adults; Analysis; Behavior; Bioassays; Competitiveness; Courtship of animals; Diapause; Fecundity; Females; Fertility; Gamma rays; Halyomorpha halys; Hatching; Identification and classification; insect pest; Insecticides; Insects; integrated pest management; Irradiation; Linear accelerators; Longevity; Males; Mating behavior; Methods; Overwintering; Parameters; Pathogens; pentatomids; Pest control; Pests; Photons; Physiological effects; Physiology; Radiation dosage; Sterility; Sterilized organisms; Stink bugs; X ray irradiation; X-ray
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects14050460

The brown marmorated stink bug, , is a pentatomid bug of Eastern Asian origin that became an economically relevant pest in the Eurasian and American continents. Management of this species is limited to use of chemical insecticides: an inefficient method due to the strong adaptability of the target pest. The sterile insect technique (SIT) is potentially a valid tactic in the search for nontoxic alternatives. In this work, we investigated the suitability of mass-trapped overwintering males, collected during the aggregation phase before the winter diapause, for their release as competitive sterile males in an SIT programme. Differently from previous studies, irradiation was applied with a linear accelerator device that produced high-energy photons. Following a similar scientific protocol with newly emerged irradiated males, the effects of X-ray irradiation on physiological parameters (longevity, fecundity and fertility) were assessed. In addition, behavioural bioassays were carried out in no-choice conditions to evaluate if irradiation interferes with mating processes. The results are very encouraging; the effects of the irradiation at 32 Gy did not differ from the controls in the longevity or fecundity of the exposed overwintering adults. The hatching rate of the eggs laid by the fertile females that had mated with the irradiated males was less than 5%. The results of behavioural bioassays showed that the irradiation did not cause a significant impact on the quality of the sterile males. More research is warranted to evaluate the mating competitiveness of sterile males in semi-field and field conditions.