Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)

[Display omitted] •Chemical modification with benzaldehyde and anisaldehyde affects only 1–2 amino groups of lysozyme molecule.•Chemical modification does not prevent further covalent immobilization of the protein using the remaining free amino groups.•Composite material based on modified covalently...

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Published in:Biotechnology reports (Amsterdam, Netherlands) Netherlands), 2019-12, Vol.24, p.e00381-e00381, Article e00381
Main Authors: Levashov, Pavel A., Matolygina, Darya A., Dmitrieva, Oxana A., Ovchinnikova, Ekaterina D., Adamova, Irina Yu, Karelina, Nataliya V., Nelyub, Vladimir A., Eremeev, Nikolay L., Levashov, Andrey V.
Format: Article
Language:English
Subjects:
Anisaldehyde
Bacteriolytic activity
Benzaldehyde
Chemically modified lysozyme
Endotoxin adsorption
Immobilized lysozyme
Immunoglobulin G adsorption
Sepsis
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Summary:[Display omitted] •Chemical modification with benzaldehyde and anisaldehyde affects only 1–2 amino groups of lysozyme molecule.•Chemical modification does not prevent further covalent immobilization of the protein using the remaining free amino groups.•Composite material based on modified covalently immobilized lysozyme shows excellent endotoxin adsorption.•The adsorption of IgG on immobilized lysozyme did not increase after chemical modification.•The ability to specifically bind to IgG may be associated with the opsonin function of lysozyme. Chemical modification of lysozyme was carried out using benzaldehyde and anisaldehyde. It was shown that chemical modification affects only 1–2 amino groups of the protein molecule which does not prevent further covalent immobilization of lysozyme using the remaining free amino groups. The bacteriolytic activity of lysozyme is preserved after chemical modification and after subsequent covalent immobilization. As a result of chemical modification immobilized lysozyme more effectively adsorbs bacterial lipopolysaccharides (endotoxins). Adsorption of immunoglobulin G does not increase after modification. The sorbents obtained in this work can be used for the future development of new medical material for the extracorporeal treatment of sepsis. The proposed scheme for the modification and immobilization of lysozyme can be used with various aldehydes for the preparation of sorbents with different properties.
ISSN:2215-017X
2215-017X
DOI:10.1016/j.btre.2019.e00381