The MKK2a Gene Involved in the MAPK Signaling Cascades Enhances Populus Salt Tolerance

Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction modules, which transmit environmental signals in plant cells through stepwise phosphorylation and play indispensable roles in a wide range of physiological and biochemical processes. Here, we isolated and chara...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2022-09, Vol.23 (17), p.10185
Main Authors: Wang, Jiali, Sun, Zimou, Chen, Caihui, Xu, Meng
Format: Article
Language:English
Subjects:
Abiotic stress
Adaptation
Amino acids
Apoptosis
Catalase
Genes
Kinases
Localization
MAP kinase
MAPK signaling cascades
Morphogenesis
Open reading frames
overexpression
Pathogens
PeMKK2a gene
Peroxidase
Phosphorylation
Plant cells
Poplar
Populus
Protein kinase C
Proteins
Salinity tolerance
Salt
salt stress
Seeds
Signal transduction
Superoxide dismutase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction modules, which transmit environmental signals in plant cells through stepwise phosphorylation and play indispensable roles in a wide range of physiological and biochemical processes. Here, we isolated and characterized a gene encoding MKK2 protein from poplar through the rapid amplification of cDNA ends (RACE). The full-length PeMKK2a gene was 1571 bp, including a 1068 bp open reading frame (ORF) encoding 355 amino acids, and the putative PeMKK2a protein belongs to the PKc_like (protein kinase domain) family (70–336 amino acids) in the PKc_MAPKK_plant subfamily and contains 62 sites of possible phosphorylation and two conserved domains, DLK and S/T-xxxxx-S/T. Detailed information about its gene structure, sequence similarities, subcellular localization, and transcript profiles under salt-stress conditions was revealed. Transgenic poplar lines overexpressing PeMKK2a exhibited higher activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) than non-transgenic poplar under salt stress conditions. These results will provide insight into the roles of MAPK signaling cascades in poplar response to salt stress.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms231710185