Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

• Published in: BMC plant biology 2024-04, Vol.24 (1), p.318-318, Article 318
• Main Authors: Gao, Wei, Jiang, Yating, Yang, Xiaohu, Li, Ting, Zhang, Litian, Yan, Shengnan, Cao, Jiajia, Lu, Jie, Ma, Chuanxi, Chang, Cheng, Zhang, Haiping
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - metabolism; Analysis; Arabidopsis - genetics; Arabidopsis - physiology; Biosynthesis; Catabolism; Class III peroxidase; Cytoplasm; Diseases and pests; Dormancy; Endoplasmic reticulum; Functional analysis; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genetic aspects; Genetic modification; Genome editing; Germination; Germination - genetics; Gibberellic acid; Gibberellins - metabolism; Growth; High temperature; Homeostasis; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Methods; Mutation; Peroxidase; Peroxidases - genetics; Peroxidases - metabolism; Plant breeding; Plant Dormancy - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; Pre-harvest sprouting; Proteins; Reactive oxygen species; Scavenging; Seed dormancy; Seeds; Seeds - genetics; Seeds - growth & development; Seeds - physiology; Transcriptomes; Transgenic plants; Triticum - enzymology; Triticum - genetics; Triticum - physiology; Wheat
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05041-4

Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive. Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using the transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 (J411) background. These results indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H O homeostasis by scavenging excess H O and participated in the biosynthesis and catabolism pathways of gibberellic acid and abscisic acid to regulate SD and germination. These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high pre-harvest sprouting resistance by gene editing technology.