Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

• Published in: Food technology and biotechnology 2021-09, Vol.59 (3), p.306-313
• Main Authors: Batista, Ryhára Dias, Melo, Fernanda Guimarães, do Amaral Santos, Claudia Cristina Auler, de Paula-Elias, Fabrício Coutinho, Perna, Rafael Firmani, Xavier, Michelle Cunha Abreu, Villalba Morales, Sergio Andres, de Almeida, Alex Fernando
• Format: Article
• Language: English
• Subjects: Agricultural wastes; Aspergillus carbonarius; Aspergillus carbonarius PC-4; b-Fructofuranosidase; culture optimization; Enzymatic activity; Enzyme activity; Enzymes; Food industry; Fructooligosaccharides; Hydrolysis; Industrial applications; Invertase; Lattice design; Microorganisms; Optimization; pH effects; Physicochemical properties; Pineapples; Scientific Note; simplex lattice design; Substrates; Sucrose; sucrose hydrolysis; Sugar; Yeasts
• ISSN: 1330-9862; 1334-2606
• DOI: 10.17113/ftb.59.03.21.6934

Microbial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated PC-4 strain was used to optimize the β-fructofuranosidase production in a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars. Optimization of nutritional components of culture medium was carried out using simplex lattice mixture design for 72 and 120 h at 28 °C. One-factor-at-a-time methodology was used to optimize the physicochemical parameters. Crude enzyme was used for sucrose hydrolysis at different concentrations. The optimized conditions of enzyme production were achieved from cultivations containing pineapple crown waste (1.3%, ) and yeast extract (0.3%, ) after 72 h with an enzyme activity of 9.4 U/mL, obtaining R =91.85%, R =85.06%, highest -value (13.52) and low p-value (0.003). One-factor-at-a-time used for optimizing the physicochemical conditions showed optimum temperature (20 °C), pH (5.5), agitation (180 rpm) and time course (72 h) with a 3-fold increase of enzyme production. The invertase-induced sucrose hydrolysis showed the maximum yield (3.45 mmol of reducing sugars) using 10% of initial sucrose concentration. Higher sucrose concentrations caused the inhibition of invertase activity, possibly due to the saturation of substrate or formation of sucrose aggregates, making it difficult for the enzyme to access sucrose molecules within the created clusters. Therefore, a cost-effective method was developed for the invertase production using agroindustrial waste and the produced enzyme can be used efficiently for inverted sugar production at high sucrose concentration. This study presents an efficient utilization of pineapple crown waste to produce invertase by a newly isolated PC-4 strain. This enzyme exhibited a good potential for inverted sugar production at high initial sucrose concentration, which is interesting for industrial applications.