Enhancing scaffold-free spheroid models: 3D cell bioprinting method for metastatic HSC3–Oral squamous carcinoma cell line

• Published in: SLAS discovery 2024-06, Vol.29 (4), p.100158-100158, Article 100158
• Main Authors: de Araújo, Taís Bacelar Sacramento, Nogueira, Raphael Luís Rocha, Siquara da Rocha, Leonardo de Oliveira, Bastos, Iasmin Nogueira, Dias, Rosane Borges, Souza, Bruno Solano De Freitas, Lambert, Daniel William, Coletta, Ricardo D., Silva, Viviane Aline Oliveira, Gurgel Rocha, Clarissa A.
• Format: Article
• Language: English
• Subjects: 3D cell culture; Bioprinting - methods; Carcinoma, Squamous Cell - pathology; Cell Culture Techniques, Three Dimensional - methods; Cell Line, Tumor; Coculture Techniques - methods; Humans; Magnetic bioprinting; Mouth Neoplasms - pathology; Oral cancer; Printing, Three-Dimensional; Protocols; Spheroids; Spheroids, Cellular - pathology
• ISSN: 2472-5552; 2472-5560
• DOI: 10.1016/j.slasd.2024.100158

3D in vitro systems offer advantages over the shortcomings of two-dimensional models by simulating the morphological and functional features of in vivo-like environments, such as cell-cell and cell-extracellular matrix interactions, as well as the co-culture of different cell types. Nevertheless, these systems present technical challenges that limit their potential in cancer research requiring cell line- and culture-dependent standardization. This protocol details the use of a magnetic 3D bioprinting method and other associated techniques (cytotoxicity assay and histological analysis) using oral squamous cell carcinoma cell line, HSC3, which offer advantages compared to existing widely used approaches. This protocol is particularly timely, as it validates magnetic bioprinting as a method for the rapid deployment of 3D cultures as a tool for compound screening and development of heterotypic cultures such as co-culture of oral squamous cell carcinoma cells with cancer-associated fibroblasts (HSC3/CAFs).