Transcriptomic Signature and Pro-Osteoclastic Secreted Factors of Abnormal Bone-Marrow Stromal Cells in Fibrous Dysplasia

• Published in: Cells (Basel, Switzerland) Switzerland), 2024-05, Vol.13 (9), p.774
• Main Authors: Michel, Zachary, Raborn, Layne N, Spencer, Tiahna, Pan, Kristen S, Martin, Daniel, Roszko, Kelly L, Wang, Yan, Robey, Pamela G, Collins, Michael T, Boyce, Alison M, de Castro, Luis Fernandez
• Format: Article
• Language: English
• Subjects: Adult; Animal models; Animals; Biomarkers; Bone marrow; Bone turnover; bone-marrow stromal cell; Bones; Cell culture; Culture media; Cyclic adenylic acid; Cyclic AMP; Cytokines; Cytokines - metabolism; Denosumab; Development and progression; Dysplasia; Ethylenediaminetetraacetic acid; Extracellular matrix; Female; Fibrous dysplasia; Fibrous Dysplasia of Bone - genetics; Fibrous Dysplasia of Bone - metabolism; Fibrous Dysplasia of Bone - pathology; Gene expression; GTP-Binding Protein alpha Subunits, Gs - genetics; GTP-Binding Protein alpha Subunits, Gs - metabolism; Health aspects; Humans; Inflammation; Male; Mesenchymal Stem Cells - metabolism; Mice; Microenvironments; Middle Aged; Osteoclasts; osteoprogenitor; Penicillin; Reagents; Secretome; skeletal stem cell; skeleton; Stromal cells; Therapeutic targets; Transcriptome - genetics; Transcriptomes; Transcriptomics
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells13090774

Fibrous dysplasia (FD) is a mosaic skeletal disorder caused by somatic activating variants of encoding for Gα and leading to excessive cyclic adenosine monophosphate signaling in bone-marrow stromal cells (BMSCs). The effect of Gα activation in the BMSC transcriptome and how it influences FD lesion microenvironment are unclear. We analyzed changes induced by Gα activation in the BMSC transcriptome and secretome. RNAseq analysis of differential gene expression of cultured BMSCs from patients with FD and healthy volunteers, and from an inducible mouse model of FD, was performed, and the transcriptomic profiles of both models were combined to build a robust FD BMSC genetic signature. Pathways related to Gα activation, cytokine signaling, and extracellular matrix deposition were identified. To assess the modulation of several key secreted factors in FD pathogenesis, cytokines and other factors were measured in culture media. Cytokines were also screened in a collection of plasma samples from patients with FD, and positive correlations of several cytokines to their disease burden score, as well as to one another and bone turnover markers, were found. These data support the pro-inflammatory, pro-osteoclastic behavior of FD BMSCs and point to several cytokines and other secreted factors as possible therapeutic targets and/or circulating biomarkers for FD.