Loading…
Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment
Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the v...
Saved in:
| Published in: | Viruses 2024-12, Vol.16 (12), p.1898 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c325t-56e1dac808178bd8f059db5d1ef3aab6d1e4abad5d4f6b19e66ba6776aa999513 |
| container_end_page | |
| container_issue | 12 |
| container_start_page | 1898 |
| container_title | Viruses |
| container_volume | 16 |
| creator | Hubbard, Laura E Stelzer, Erin A Poulson, Rebecca L Kolpin, Dana W Szablewski, Christine M Givens, Carrie E |
| description | Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protocol was dead-end ultrafiltration coupled with salt solution elution and centrifugation concentration. Using this method, infectious virus was recovered at 1 × 10
50% egg infectious dose per milliliter (EID
/mL), whereas viral RNA was detected inconsistently down to 1 × 10
EID
/mL. This method will aid in furthering our understanding of the avian influenza virus in the environment and may be applicable to the environmental detection of other enveloped viruses. |
| doi_str_mv | 10.3390/v16121898 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_d85f5a2c591a4b2fa9134584ba11761f</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_d85f5a2c591a4b2fa9134584ba11761f</doaj_id><sourcerecordid>3149763236</sourcerecordid><originalsourceid>FETCH-LOGICAL-c325t-56e1dac808178bd8f059db5d1ef3aab6d1e4abad5d4f6b19e66ba6776aa999513</originalsourceid><addsrcrecordid>eNpdkk1v1DAQQCMEoqVw4A8gS1zKIZCJY8c-odXSwkqLkBD0ak2S8W5Wib11PiTgz9fLllXLyaOZp-eZ0STJa8jec66zDzNIyEFp9SQ5B611WmgQTx_EZ8mLYdhlmZQ6K58nZ1yXZZ5n4jz584lm6vy-JzcybxmyNYYNpTe-m3piS-_qWAk4tt6xrzRufcNGz75T7WcKbOUs1bE2DWwxt-gOiW4i9xvZTRti1gbfs3FLbHE7RUnNrtzcBu8O_71MnlnsBnp1_14kP6-vfiy_pOtvn1fLxTqteS7GVEiCBmuVKShV1SibCd1UogGyHLGSMSiwwkY0hZUVaJKyQlmWEjHOL4BfJKujt_G4M_vQ9hh-GY-t-ZvwYWMwxN46Mo0SVmBeCw1YVLlFDbwQqqgQoJRgo-vj0bWfqp6a43K6R9LHFdduzcbPBkCqrIA8Gi7vDcHfTjSMpm-HmroOHcU9Gg6CqxJ0cUDf_ofu_BRc3FWkCl1KnnMZqXdHqg5-GALZUzeQmcN9mNN9RPbNw_ZP5L-D4HdJB7dk</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3149763236</pqid></control><display><type>article</type><title>Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment</title><source>Publicly Available Content Database</source><source>PubMed</source><source>Coronavirus Research Database</source><creator>Hubbard, Laura E ; Stelzer, Erin A ; Poulson, Rebecca L ; Kolpin, Dana W ; Szablewski, Christine M ; Givens, Carrie E</creator><creatorcontrib>Hubbard, Laura E ; Stelzer, Erin A ; Poulson, Rebecca L ; Kolpin, Dana W ; Szablewski, Christine M ; Givens, Carrie E</creatorcontrib><description>Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protocol was dead-end ultrafiltration coupled with salt solution elution and centrifugation concentration. Using this method, infectious virus was recovered at 1 × 10
50% egg infectious dose per milliliter (EID
/mL), whereas viral RNA was detected inconsistently down to 1 × 10
EID
/mL. This method will aid in furthering our understanding of the avian influenza virus in the environment and may be applicable to the environmental detection of other enveloped viruses.</description><identifier>ISSN: 1999-4915</identifier><identifier>EISSN: 1999-4915</identifier><identifier>DOI: 10.3390/v16121898</identifier><identifier>PMID: 39772205</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Aquatic birds ; Aquatic environment ; Avian flu ; avian influenza ; Birds - virology ; Centrifugation ; Elution ; environment ; Flocculation ; infectious ; Influenza ; Influenza A virus - genetics ; Influenza A virus - isolation & purification ; Influenza in Birds - virology ; method ; Methods ; RNA, Viral - genetics ; Surface water ; Testing laboratories ; Ultrafiltration ; virus ; Viruses ; water ; Water Microbiology ; Wetlands</subject><ispartof>Viruses, 2024-12, Vol.16 (12), p.1898</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c325t-56e1dac808178bd8f059db5d1ef3aab6d1e4abad5d4f6b19e66ba6776aa999513</cites><orcidid>0000-0002-3529-6505 ; 0000-0002-8087-3295 ; 0000-0003-3813-1500 ; 0000-0001-7645-7603</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3149763236/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3149763236?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25730,27900,27901,36988,36989,38492,43870,44565,53765,53767,74381,75095</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39772205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hubbard, Laura E</creatorcontrib><creatorcontrib>Stelzer, Erin A</creatorcontrib><creatorcontrib>Poulson, Rebecca L</creatorcontrib><creatorcontrib>Kolpin, Dana W</creatorcontrib><creatorcontrib>Szablewski, Christine M</creatorcontrib><creatorcontrib>Givens, Carrie E</creatorcontrib><title>Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment</title><title>Viruses</title><addtitle>Viruses</addtitle><description>Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protocol was dead-end ultrafiltration coupled with salt solution elution and centrifugation concentration. Using this method, infectious virus was recovered at 1 × 10
50% egg infectious dose per milliliter (EID
/mL), whereas viral RNA was detected inconsistently down to 1 × 10
EID
/mL. This method will aid in furthering our understanding of the avian influenza virus in the environment and may be applicable to the environmental detection of other enveloped viruses.</description><subject>Animals</subject><subject>Aquatic birds</subject><subject>Aquatic environment</subject><subject>Avian flu</subject><subject>avian influenza</subject><subject>Birds - virology</subject><subject>Centrifugation</subject><subject>Elution</subject><subject>environment</subject><subject>Flocculation</subject><subject>infectious</subject><subject>Influenza</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza in Birds - virology</subject><subject>method</subject><subject>Methods</subject><subject>RNA, Viral - genetics</subject><subject>Surface water</subject><subject>Testing laboratories</subject><subject>Ultrafiltration</subject><subject>virus</subject><subject>Viruses</subject><subject>water</subject><subject>Water Microbiology</subject><subject>Wetlands</subject><issn>1999-4915</issn><issn>1999-4915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>COVID</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkk1v1DAQQCMEoqVw4A8gS1zKIZCJY8c-odXSwkqLkBD0ak2S8W5Wib11PiTgz9fLllXLyaOZp-eZ0STJa8jec66zDzNIyEFp9SQ5B611WmgQTx_EZ8mLYdhlmZQ6K58nZ1yXZZ5n4jz584lm6vy-JzcybxmyNYYNpTe-m3piS-_qWAk4tt6xrzRufcNGz75T7WcKbOUs1bE2DWwxt-gOiW4i9xvZTRti1gbfs3FLbHE7RUnNrtzcBu8O_71MnlnsBnp1_14kP6-vfiy_pOtvn1fLxTqteS7GVEiCBmuVKShV1SibCd1UogGyHLGSMSiwwkY0hZUVaJKyQlmWEjHOL4BfJKujt_G4M_vQ9hh-GY-t-ZvwYWMwxN46Mo0SVmBeCw1YVLlFDbwQqqgQoJRgo-vj0bWfqp6a43K6R9LHFdduzcbPBkCqrIA8Gi7vDcHfTjSMpm-HmroOHcU9Gg6CqxJ0cUDf_ofu_BRc3FWkCl1KnnMZqXdHqg5-GALZUzeQmcN9mNN9RPbNw_ZP5L-D4HdJB7dk</recordid><startdate>20241210</startdate><enddate>20241210</enddate><creator>Hubbard, Laura E</creator><creator>Stelzer, Erin A</creator><creator>Poulson, Rebecca L</creator><creator>Kolpin, Dana W</creator><creator>Szablewski, Christine M</creator><creator>Givens, Carrie E</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3529-6505</orcidid><orcidid>https://orcid.org/0000-0002-8087-3295</orcidid><orcidid>https://orcid.org/0000-0003-3813-1500</orcidid><orcidid>https://orcid.org/0000-0001-7645-7603</orcidid></search><sort><creationdate>20241210</creationdate><title>Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment</title><author>Hubbard, Laura E ; Stelzer, Erin A ; Poulson, Rebecca L ; Kolpin, Dana W ; Szablewski, Christine M ; Givens, Carrie E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-56e1dac808178bd8f059db5d1ef3aab6d1e4abad5d4f6b19e66ba6776aa999513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Aquatic birds</topic><topic>Aquatic environment</topic><topic>Avian flu</topic><topic>avian influenza</topic><topic>Birds - virology</topic><topic>Centrifugation</topic><topic>Elution</topic><topic>environment</topic><topic>Flocculation</topic><topic>infectious</topic><topic>Influenza</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza in Birds - virology</topic><topic>method</topic><topic>Methods</topic><topic>RNA, Viral - genetics</topic><topic>Surface water</topic><topic>Testing laboratories</topic><topic>Ultrafiltration</topic><topic>virus</topic><topic>Viruses</topic><topic>water</topic><topic>Water Microbiology</topic><topic>Wetlands</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hubbard, Laura E</creatorcontrib><creatorcontrib>Stelzer, Erin A</creatorcontrib><creatorcontrib>Poulson, Rebecca L</creatorcontrib><creatorcontrib>Kolpin, Dana W</creatorcontrib><creatorcontrib>Szablewski, Christine M</creatorcontrib><creatorcontrib>Givens, Carrie E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Viruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hubbard, Laura E</au><au>Stelzer, Erin A</au><au>Poulson, Rebecca L</au><au>Kolpin, Dana W</au><au>Szablewski, Christine M</au><au>Givens, Carrie E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment</atitle><jtitle>Viruses</jtitle><addtitle>Viruses</addtitle><date>2024-12-10</date><risdate>2024</risdate><volume>16</volume><issue>12</issue><spage>1898</spage><pages>1898-</pages><issn>1999-4915</issn><eissn>1999-4915</eissn><abstract>Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protocol was dead-end ultrafiltration coupled with salt solution elution and centrifugation concentration. Using this method, infectious virus was recovered at 1 × 10
50% egg infectious dose per milliliter (EID
/mL), whereas viral RNA was detected inconsistently down to 1 × 10
EID
/mL. This method will aid in furthering our understanding of the avian influenza virus in the environment and may be applicable to the environmental detection of other enveloped viruses.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39772205</pmid><doi>10.3390/v16121898</doi><orcidid>https://orcid.org/0000-0002-3529-6505</orcidid><orcidid>https://orcid.org/0000-0002-8087-3295</orcidid><orcidid>https://orcid.org/0000-0003-3813-1500</orcidid><orcidid>https://orcid.org/0000-0001-7645-7603</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1999-4915 |
| ispartof | Viruses, 2024-12, Vol.16 (12), p.1898 |
| issn | 1999-4915 1999-4915 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_d85f5a2c591a4b2fa9134584ba11761f |
| source | Publicly Available Content Database; PubMed; Coronavirus Research Database |
| subjects | Animals Aquatic birds Aquatic environment Avian flu avian influenza Birds - virology Centrifugation Elution environment Flocculation infectious Influenza Influenza A virus - genetics Influenza A virus - isolation & purification Influenza in Birds - virology method Methods RNA, Viral - genetics Surface water Testing laboratories Ultrafiltration virus Viruses water Water Microbiology Wetlands |
| title | Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-24T10%3A18%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20Large-Volume%20Concentration%20Method%20to%20Recover%20Infectious%20Avian%20Influenza%20Virus%20from%20the%20Aquatic%20Environment&rft.jtitle=Viruses&rft.au=Hubbard,%20Laura%20E&rft.date=2024-12-10&rft.volume=16&rft.issue=12&rft.spage=1898&rft.pages=1898-&rft.issn=1999-4915&rft.eissn=1999-4915&rft_id=info:doi/10.3390/v16121898&rft_dat=%3Cproquest_doaj_%3E3149763236%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c325t-56e1dac808178bd8f059db5d1ef3aab6d1e4abad5d4f6b19e66ba6776aa999513%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3149763236&rft_id=info:pmid/39772205&rfr_iscdi=true |