Potassium bromate disrupts mitochondrial distribution within murine oocytes during in vitro maturation

Purpose As disturbed mitochondrial distribution is thought to be a cause of the aging of oocytes, it was investigated whether oxidizing agents exert harmful effects on nuclear maturation and mitochondrial cluster formation in murine oocytes and whether antioxidants could rescue such harmful effects...

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Published in:Reproductive medicine and biology 2018-04, Vol.17 (2), p.143-148
Main Authors: Yamada, Kenichi, Hiradate, Yuuki, Goto, Mei, Nishiyama, Chiho, Hara, Kenshiro, Yoshida, Hiroaki, Tanemura, Kentaro
Format: Article
Language:English
Subjects:
aged oocytes
Aging
antioxidant
Antioxidants
Cancer
Experiments
Food additives
Gonadotropins
In vitro fertilization
in vitro maturation
Laboratory animals
Maturation
Metaphase
Mice
Mitochondria
Oncology, Experimental
Oocytes
Original
Oxidative stress
Oxidizing agents
Pituitary (anterior)
Potassium
Potassium bromate
Resveratrol
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Summary:Purpose As disturbed mitochondrial distribution is thought to be a cause of the aging of oocytes, it was investigated whether oxidizing agents exert harmful effects on nuclear maturation and mitochondrial cluster formation in murine oocytes and whether antioxidants could rescue such harmful effects in vitro. Methods Oocytes were obtained from female Institute of Cancer Research mice 48 h after an intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin. The oocytes were cultured with potassium bromate, an oxidizing agent, in the presence or absence of the antioxidant, resveratrol. After 12 h, the nuclear phases and mitochondrial distribution were observed. Results Significantly decreased rates of metaphase II (MII) oocytes were observed with 750 μM and 1000 μM of potassium bromate, while a significant increase in abnormal mitochondrial clusters was induced at 500 μM, 750 μM, and 1,000 μM. The addition of 10 μM or 20 μM resveratrol improved both MII maturity and the cluster formation rates in the presence of potassium bromate. Conclusions The addition of potassium bromate reduced MII maturity rates and induced abnormal mitochondrial cluster formation. This effect was alleviated by the antioxidant, resveratrol. The in vitro model used herein is useful for investigating the functions of antioxidants in the aging of oocytes.
ISSN:1445-5781
1447-0578
DOI:10.1002/rmb2.12079