Hyaluronan/Poly-L-lysine/Berberine Nanogels for Impaired Wound Healing

Physiological wound healing process can be delayed in the presence of certain pathologies, such as diabetes or cancer. In this perspective, the aim of this study was to design a new nanogel platform of hyaluronan, poly-L-lysine and berberine suitable for wound treatment. Two different nanogel formul...

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Bibliographic Details
Published in:Pharmaceutics 2020-12, Vol.13 (1), p.34
Main Authors: Amato, Giovanni, Grimaudo, Maria Aurora, Alvarez-Lorenzo, Carmen, Concheiro, Angel, Carbone, Claudia, Bonaccorso, Angela, Puglisi, Giovanni, Musumeci, Teresa
Format: Article
Language:English
Subjects:
Antimicrobial agents
Antioxidants
Aqueous solutions
Bacteria
Bacterial infections
berberine
chronic wounds
Design
Drug delivery systems
Enzymes
Extracellular matrix
hyaluronan
Hyaluronic acid
Hydration
Hydrogels
Inflammation
Kinases
nanogels
Nanoparticles
Oxidative stress
Particle size
Polymers
Skin
Wound healing
ε-polylysine
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Summary:Physiological wound healing process can be delayed in the presence of certain pathologies, such as diabetes or cancer. In this perspective, the aim of this study was to design a new nanogel platform of hyaluronan, poly-L-lysine and berberine suitable for wound treatment. Two different nanogel formulations were selected after a first formulation screening. They were prepared by adding dropwise 2 mg/mL hyaluronan aqueous solution (200 or 700 kDa) to 1.25 mg/mL poly-L-lysine aqueous solution. Blank nanogels formulated with 200 kDa HA resulted stable after freeze-drying with dimensions, polydispersity index and zeta potential of 263.6 ± 13.1 nm, 0.323 ± 0.029 and 32.7 ± 3.5 mV, respectively. Both blank and berberine-loaded nanogels showed rounded-shape structures. Loaded nanogels released nearly 50% of loaded berberine within 45 min, whereas the remaining 50% was released up to 24 h in vitro. Both, blank and berberine-loaded nanogels were able to completely close the fibroblasts gap in 42 h.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics13010034