Involvement of 2′-5′ oligoadenylate synthetase-like protein in the survival of Mycobacterium tuberculosis avirulent strain in macrophages

Mycobacterium tuberculosis ( M. tuberculosis ) can replicate in the macrophage by interfering with many host protein functions. While it is far from known these host proteins for controlling M. tuberculosis infection. Herein, we infected macrophages including THP-1 and Raw264.7 cells with M. tubercu...

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Published in:Animal diseases 2023-03, Vol.3 (1), p.5-11, Article 5
Main Authors: Reheman, Aikebaier, Cao, Xiaojian, Wang, Yifan, Nie, Xi, Cao, Gang, Zhou, Wei, Yang, Bing, Lei, Yingying, Zhang, Weipan, Naeem, Muhammad Ahsan, Chen, Xi
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Bacteria
Bioinformatics
Experiments
Gene expression
Health Promotion and Disease Prevention
Immune system
Infections
Infectious Diseases
Interferon
Interferon stimulated genes
Macrophages
Medicine
Medicine & Public Health
Metabolic Diseases
Mycobacterium tuberculosis
OASL
Original Article
Pathogens
Proteins
Signal transduction
Survival
Tuberculosis
Veterinary Medicine/Veterinary Science
Yeasts
Zoonoses
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Summary:Mycobacterium tuberculosis ( M. tuberculosis ) can replicate in the macrophage by interfering with many host protein functions. While it is far from known these host proteins for controlling M. tuberculosis infection. Herein, we infected macrophages including THP-1 and Raw264.7 cells with M. tuberculosis and identified the differentially expressed genes (DEGs) in the interferon signaling pathway. Among them, 2′-5′ oligoadenylate synthetase-like (OASL) underwent the greatest upregulation in M. tuberculosis -infected macrophages. Knockdown of the expression of OASL attenuated M. tuberculosis survival in macrophages. Further, bioinformatics analysis revealed the potential interaction axis of OASL-TAB3- Rv0127, which was further validated by the yeast-two-hybrid (Y2H) assay and Co-IP. This interaction axis might regulate the M. tuberculosis survival and proliferation in macrophages. The study reveals a possible role of OASL during M. tuberculosis infection as a target to control its propagation.
ISSN:2731-0442
2731-0442
DOI:10.1186/s44149-023-00068-w