Energetic costs regulated by cell mechanics and confinement are predictive of migration path during decision-making

• Published in: Nature communications 2019-09, Vol.10 (1), p.4185-12, Article 4185
• Main Authors: Zanotelli, Matthew R., Rahman-Zaman, Aniqua, VanderBurgh, Jacob A., Taufalele, Paul V., Jain, Aadhar, Erickson, David, Bordeleau, Francois, Reinhart-King, Cynthia A.
• Format: Article
• Language: English
• Subjects: 13; 14; 14/19; 14/3; 14/63; 142/126; 631/80/84/750; 639/166/985; Adenosine Diphosphate - metabolism; Adenosine Triphosphate - metabolism; Biomedical Engineering; Cancer; Caveolin 1 - genetics; Caveolin 1 - metabolism; Cell adhesion & migration; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell Movement - physiology; Confined spaces; Confinement; Cost assessments; Costs; Decision Making; Decision theory; Glucose - metabolism; Humanities and Social Sciences; Humans; Metabolism; Metastases; Metastasis; Microscopy, Atomic Force; Microscopy, Confocal; Microscopy, Phase-Contrast; multidisciplinary; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Science; Science (multidisciplinary); Stiffness
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-12155-z

Cell migration during the invasion-metastasis cascade requires cancer cells to navigate a spatially complex microenvironment that presents directional choices to migrating cells. Here, we investigate cellular energetics during migration decision-making in confined spaces. Theoretical and experimental data show that energetic costs for migration through confined spaces are mediated by a balance between cell and matrix compliance as well as the degree of spatial confinement to direct decision-making. Energetic costs, driven by the cellular work needed to generate force for matrix displacement, increase with increasing cell stiffness, matrix stiffness, and degree of spatial confinement, limiting migration. By assessing energetic costs between possible migration paths, we can predict the probability of migration choice. Our findings indicate that motility in confined spaces imposes high energetic demands on migrating cells, and cells migrate in the direction of least confinement to minimize energetic costs. Therefore, therapeutically targeting metabolism may limit cancer cell migration and metastasis. Migrating cells tune their energy utilization in response to their microenvironment, but how cellular energetics direct navigation remains unclear. Here, the authors report that energetic costs for motility, regulated by cell mechanics and confinement, predict the probability of migration choice.