A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties

• Published in: International journal of nanomedicine 2017-01, Vol.12, p.3665-3679
• Main Authors: Ma, Kena, Gong, Lingling, Cai, Xinjie, Huang, Pin, Cai, Jing, Huang, Dan, Jiang, Tao
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - adverse effects; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antimicrobial agents; Bacteria; Biofilms; Bond strength; Cell cycle; Cell Line; chitosan; Chitosan - chemistry; Cytotoxicity; Drug resistance; EPD; Escherichia coli - drug effects; gelatin; Gelatin - chemistry; Green Chemistry Technology; Health aspects; Infections; Laboratories; Mice; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; nanocomposite coatings; Nanocomposites; Nanocomposites - chemistry; Nanocomposites - toxicity; Nanocomposites - ultrastructure; Nanoparticles; Nanoparticles - ultrastructure; Nitrates; Original Research; Physiological aspects; Properties; silver; Silver - chemistry; Staphylococcus aureus - drug effects; Studies; Surgery; Titanium; Toxicity; Transplants & implants
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S130857

Implant-associated infections still pose a serious threat leading to several complications. This study reported an environmentally benign Ag-containing nanocomposite coating with efficient antibacterial property fabricated on the metal implant via electrophoretic deposition (EPD). In such coatings, Ag O/AgCl mixed with chitosan/gelatin (CS/G) polymers work together to exert the antibacterial property which could act as an alternative to traditional Ag nanoparticles. Scanning electron microscopy images showed the shuttle fiber-like morphology distributed lamellarly and some nanoparticles carved uniformly into the cross section. Transmission electron microscopy results revealed a core-shell-like structure of the released nanoparticles in experimental groups. The Ag-containing coatings exhibited strong antibacterial properties against strains and strains. Meanwhile, the CCK-8 tests showed that after assembling with chitosan and gelatin polymers, the cytotoxicity of Ag was largely decreased. In addition, such coatings also exhibited strong bond strength with metal substrates and good degradable properties. Therefore, such Ag-containing CS/G coatings fabricated via EPD may be a promising candidate to be administrated in controlling the implant-associated infections.