Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration

Apical periodontitis (AP) causes arrest of tooth root development, which is associated with impaired odontoblastic differentiation of stem cells from apical papilla (SCAPs), but the underlying mechanism remains unclear. Here, we investigated roles of extracellular vesicle (EV) in AP and odontoblasti...

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Bibliographic Details
Published in:Journal of tissue engineering 2022-03, Vol.13, p.20417314221084095-20417314221084095
Main Authors: Yang, Shengyan, Liu, Qing, Chen, Shijing, Zhang, Fuping, Li, Yaoyin, Fan, Wenguo, Mai, Lijia, He, Hongwen, Huang, Fang
Format: Article
Language:English
Subjects:
CD63 antigen
Cell differentiation
Cell proliferation
Dental pulp
Dentin
Dentinogenesis
Differentiation
Encapsulation
Extracellular vesicles
Gum disease
In vivo methods and tests
Inflammation
Lipopolysaccharides
Nuclear factor I
Original
Periodontitis
Regeneration
Root development
Stem cells
Teeth
Tissue engineering
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Summary:Apical periodontitis (AP) causes arrest of tooth root development, which is associated with impaired odontoblastic differentiation of stem cells from apical papilla (SCAPs), but the underlying mechanism remains unclear. Here, we investigated roles of extracellular vesicle (EV) in AP and odontoblastic differentiation of SCAPs, moreover, a novel nuclear factor I/C (NFIC)-encapsulated EV was developed to promote dentin regeneration. We detected a higher expression of EV marker CD63 in inflamed apical papilla, and found that EVs from LPS-stimulated dental pulp cells suppressed odontoblastic differentiation of SCAPs through downregulating NFIC. Furthermore, we successfully constructed the NFIC-encapsulated EV by overexpressing NFIC in HEK293FT cells, which could upregulate cellular NFIC level in SCAPs, promoting the proliferation and migration of SCAPs, as well as dentinogenesis both in vitro and in vivo. Collectively, based on pathological roles of EV in AP, our study provides a novel strategy for dentin regeneration by exploiting EV to deliver NFIC.
ISSN:2041-7314
2041-7314
DOI:10.1177/20417314221084095