Proteomics discovery of pulmonary hypertension biomarkers: Insulin‐like growth factor binding proteins are associated with disease severity

Pulmonary arterial hypertension (PAH) is a progressive disease characterized by sustained elevations of pulmonary artery pressure. To date, we lack circulating, diagnostic, and prognostic markers that correlate to clinical and functional parameters. In this study, we performed mass spectrometry‐base...

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Published in:Pulmonary circulation 2022-04, Vol.12 (2), p.e12039-n/a
Main Authors: Nies, Melanie K., Yang, Jun, Griffiths, Megan, Damico, Rachel, Zhu, Jie, Vaydia, Dhananjay, Fu, Zongming, Brandal, Stephanie, Austin, Eric D., Ivy, Dunbar D., Hassoun, Paul M., Van Eyk, Jennifer E., Everett, Allen D.
Format: Article
Language:English
Subjects:
biomarker
Biomarkers
Growth factors
IGF binding protein
Plasma
Proteins
Proteomics
Pulmonary arteries
Pulmonary hypertension
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Summary:Pulmonary arterial hypertension (PAH) is a progressive disease characterized by sustained elevations of pulmonary artery pressure. To date, we lack circulating, diagnostic, and prognostic markers that correlate to clinical and functional parameters. In this study, we performed mass spectrometry‐based proteomics analysis to identify circulating biomarkers of PAH. Plasma samples from patients with idiopathic pulmonary arterial hypertension (IPAH, N = 9) and matched normal controls (N = 9) were digested with trypsin and analyzed using data‐dependent acquisition on an Orbitrap mass spectrometer. A total of 826 (false discovery rate [FDR] 0.047) and 461 (FDR 0.087) proteins were identified across all plasma samples obtained from IPAH and control subjects, respectively. Of these, 153 proteins showed >2 folds change (p 
ISSN:2045-8940
2045-8932
2045-8940
DOI:10.1002/pul2.12039