Differences between inflammatory cells infiltrated into tunica intima, media, and adventitia of ascending aortic aneurysms within diabetic and hypertensive patients

• Published in: Biomolecules & biomedicine 2023-08, Vol.23 (4), p.596-604
• Main Authors: Milutinović, Aleksandra, Zivin, Marko, Zorc-Pleskovič, Ruda
• Format: Article
• Language: English
• Subjects: Adventitia; Aneurysm, Ascending Aorta; Aortic Aneurysm - pathology; arterial hypertension (AH); ascending aortic aneurysm; Diabetes Mellitus, Type 2 - complications; Humans; Hypertension - complications; intima; media; Tunica Intima - chemistry; type 2 diabetes (DM)
• ISSN: 2831-0896; 2831-090X
• DOI: 10.17305/bb.2022.8565

The risk factors that are the most significant for the development of most cardiovascular diseases are arterial hypertension (AH), type 2 diabetes (DM), and inflammation. However, for the development of aortic aneurysms, DM is not one of them. Our study aimed to evaluate the difference between inflammatory infiltration in three individual layers of the ascending aortic aneurysm within diabetic and hypertensive patients. Forty-five patients aged 36 to 80 were divided into a group with diabetic patients without AH (group DM, N=8) and hypertensive patients without DM (group AH, N=37). For the histological analysis, aortic aneurysms were stained with hematoxylin eosin and Movat. We used immunochemical methods to detect pro- (M1), anti-inflammatory (M2) macrophages, T-helper, T-killer cells, B cells, and plasma cells. Statistical analysis was done by independent-samples Kruskal-Wallis test adjusted by Bonferroni correction for multiple tests (P