The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population

Bone tissue is constantly regenerated and repaired through a finely balanced process known as bone remodeling. Many miRNAs act as regulators of the signaling pathways involved in bone metabolic processes to maintain tissue homeostasis. This study aimed to assess the circulating levels of MIR21, MIR3...

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Bibliographic Details
Published in:Scientific reports 2024-11, Vol.14 (1), p.27779-14, Article 27779
Main Authors: El-Tahan, Rasha A, Oriquat, Ghaleb A, Sorour, Islam, Salem, Sherif M, Kamel, Maher A, Michel, Trez N, Abu-Samra, Nehal
Format: Article
Language:English
Subjects:
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Adult
Alkaline phosphatase
Alkaline Phosphatase - blood
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Biomarkers - blood
Bone and Bones - metabolism
Bone metabolism
Bone quality
Bone remodeling
Bone Remodeling - genetics
Bone turnover
Decompression
Egypt
Egyptian population
Enzyme-linked immunosorbent assay
Female
Gene Expression Regulation
Homeostasis
Humanities and Social Sciences
Humans
Male
MicroRNA
MicroRNAs
MicroRNAs - blood
MicroRNAs - genetics
Middle Aged
miRNA
multidisciplinary
Osteocalcin
Osteocalcin - blood
Osteocalcin - genetics
Population studies
Prospective Studies
Science
Science (multidisciplinary)
Serum levels
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Summary:Bone tissue is constantly regenerated and repaired through a finely balanced process known as bone remodeling. Many miRNAs act as regulators of the signaling pathways involved in bone metabolic processes to maintain tissue homeostasis. This study aimed to assess the circulating levels of MIR21, MIR34a, and MIR155 in human serum and their bone expression, and the expression of bone turnover-related genes which can reflect the bone quality. This prospective study was conducted on 60 patients (30 males and 30 females) indicated for surgical interventions for neural decompression +/- fixation. Relative quantification of expression of MIR21, miR34a, and MIR155 and bone related genes was assayed using PCR. The serum levels of osteocalcin and Serum Bone Alkaline Phosphatase (sBAP) were assayed using a human ELISA kit. The main finding of the present work was the strong positive association between the circulating levels of only miR21 and MIR155 and their bone expression in the population under study and with bone markers and target genes Also, a positive association was found between both bone expression and circulating MIR21 levels with age and sBAP. These results suggest that the circulating levels of these microRNAs as early markers for the predication of bone quality.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-77643-9