Comparison of the lymphocyte response to interval exercise versus continuous exercise in recreationally trained men

• Published in: Brain, behavior, & immunity. Health behavior, & immunity. Health, 2022-03, Vol.20, p.100415-100415, Article 100415
• Main Authors: Arroyo, Eliott, Tagesen, Emily C., Hart, Tricia L., Miller, Brandon A., Jajtner, Adam R.
• Format: Article
• Language: English
• Subjects: Aerobic exercise; B cells; Full Length; HIIT; NK cells; Sprint interval exercise; T cells
• ISSN: 2666-3546; 2666-3546
• DOI: 10.1016/j.bbih.2022.100415

The purpose of this investigation was to compare changes in circulating lymphocyte subset cell counts between high-intensity interval exercise (HIIE), sprint interval exercise (SIE), and moderate-intensity continuous exercise (MICE). Recreationally active men (n ​= ​11; age: 23 ​± ​4 ​yr; height: 179.9 ​± ​4.5 ​cm; body mass: 79.8 ​± ​8.7 ​kg; body fat %:12.6 ​± ​3.8%; V̇O2max: 46.6 ​± ​3.9 ​ml⋅kg−1⋅min−1) completed a maximal graded exercise test to determine maximal oxygen uptake (V̇O2max) and three duration-matched cycling trials (HIIE, SIE, and MICE) in a randomized, counterbalanced fashion. HIIE consisted of fifteen 90-s bouts at 85% V̇O2max interspersed with 90-s active recovery periods. SIE consisted of fifteen 20-s bouts at 130% maximal power and 160-s active recovery periods. MICE was a continuous bout at 65% V̇O2max. Total exercise duration was 53 ​min in all three trials, including warm-up and cool-down. Blood was collected before, immediately post, 30 ​min, 2 ​h, 6 ​h, and 24 ​h post-exercise. Changes in lymphocyte subset counts, and surface expression of various markers were analyzed via flow cytometry. Changes were assessed using mixed model regression analysis with an autoregressive first order repeated measures correction. Significant decreases were observed in absolute counts of CD56dim NK cells, CD19+ B cells, CD4+ T cells, and CD8+ T cells 30 ​min and 24-h post-exercise in all three trials. Despite resulting in greater total work and oxygen consumption, MICE elicited similar changes in lymphocyte subset counts and receptor expression compared to both SIE and HIIE. Similarly, while the two interval trials resulted in differing oxygen consumption and total work, no differences in the lymphocyte response were observed. Though both forms of exercise resulted in declines in circulating lymphocyte cell counts, neither exercise type provides an immune-related advantage when matched for duration. •Continuous and interval exercise elicit similar changes in lymphocyte counts.•Sprint and high-intensity interval exercise elicit similar lymphocyte responses.•B, NK, and T cell subset counts are blunted 30 ​min and 24 ​h post-exercise.•Acute exercise increases the expression cell migration markers on CD8+ T cells.•Acute exercise may impair CD56dim NK cell function measured by CD69 expression.