Optimization of the arginase activity assay micromethod for macrophages and sera

We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke's method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice,...

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Bibliographic Details
Published in:BMC research notes 2023-08, Vol.16 (1), p.1-188, Article 188
Main Authors: Nzoumbou-Boko, Romaric, Zolipou, Cyrille Oliver Ozzin-Kholy, Yambiyo, Brice Martial, Semballa, Silla, Nalingbo, Mireille Cornelia Ingrid Denissio Morissi, Daulouède, Sylvie, Vincendeau, Philippe
Format: Article
Language:English
Subjects:
African trypanosomiasis
Analysis
Arginase
Arginase activity
Care and treatment
Comparative analysis
COVID-19
Diagnosis
Enzymes
Immune response
Infections
Laboratory animals
Liver function tests
Lysates
Macrophages
Metabolism
Micromethod optimization
Micromethods
Parasites
Parasitic diseases
Physiology
Physiology, Pathological
Polyamines
Research Note
Spectrophotometry
Tropical diseases
Trypanosomiasis
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Summary:We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke's method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice, and in purified bovine liver arginase was compared using the conventional and optimized micromethods. The sensitivity of both micromethods was comparable. However, our optimized method has the following advantages: it uses small sample volumes (6 [micro]l per assay vs. 50 [micro]l) and reagent volumes (200 [micro]l vs. 400 [micro]l), it can be carried out in a single microplate well, thereby minimizing handling, and it requires fewer materials and utilizes readily available equipment. Our optimized method proved to be applicable and well suited for small-volume samples and resource-poor laboratories.
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-023-06462-4