Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insu...

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Bibliographic Details
Published in:Journal of biological engineering 2020-08, Vol.14 (1), p.23-23, Article 23
Main Authors: Kuncorojakti, Suryo, Rodprasert, Watchareewan, Yodmuang, Supansa, Osathanon, Thanaphum, Pavasant, Prasit, Srisuwatanasagul, Sayamon, Sawangmake, Chenphop
Format: Article
Language:English
Subjects:
Alginate
Alginates
Alginic acid
Analysis
Cell adhesion & migration
Dental pulp
Dental pulp stem cells (DPSCs)
Dextrose
Diabetes
Diabetes mellitus
Diabetes therapy
Encapsulation
Endoderm
Experiments
Functional analysis
Gene expression
Glucose
Glucose diffusion
Insulin
Insulin-producing cells (IPCs)
Markers
Metabolism
Nkx6.1 protein
Pancreas
Pluronic F127
Poloxamers
Stem cells
Transplantation
Viability
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Summary:Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp-derived stem cell (hDPSC)-derived IPCs by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG and ALGPA-encapsulated hDPSC-derived IPCs maintained viability for at least 336 h and sustained pancreatic endoderm marker ), pancreatic islet markers ( and ), and intracellular pro-insulin and insulin expressions for at least 14 days. Functional analysis revealed a glucose-responsive C-peptide secretion of ALG- and ALGPA-encapsulated hDPSC-derived IPCs at 14 days post-encapsulation. ALG and ALGPA encapsulations efficiently preserved the viability and functionality of hDPSC-derived IPCs in vitro and could be the potential transplantation platform for further clinical application.
ISSN:1754-1611
1754-1611
DOI:10.1186/s13036-020-00246-1