Synthesis, molecular docking, and anti-breast cancer study of 1-H-indol-3-Carbohydrazide and their derivatives

• Published in: Results in Chemistry 2024-10, Vol.11, p.101762, Article 101762
• Main Authors: Mahdi, Zainab H., Alsalim, Tahseen A., Abdulhussein, Heider A., Majed, Ahmed A., Abbas, Sabah
• Format: Article
• Language: English
• Subjects: Anticancer; CDK4; Indole derivatives; MCF-7; Molecular docking; Pharmacokinetic properties; Triazol
• ISSN: 2211-7156; 2211-7156
• DOI: 10.1016/j.rechem.2024.101762

[Display omitted] A prominent heterocyclic system found in medications and natural goods is indoles. Due to the significance of this important ring system, a fresh batch of unique indole derivatives was created using indole carbohydrazide. The triazole moiety of indole derivative (3) is produced by reacting carbohydrazide with carbon disulfide in alkaline conditions, followed by hydrazine treatment. In addition to synthesizing additional derivatives, indole-bearing triazole (3) was condensed with aromatic aldehydes to create the indole derivatives (4a–c). Mass spectra, 1HNMR, 13CNMR, and infrared spectroscopy were used to diagnose all produced derivatives. An MTT assay was employed to test the derivatives against MCF-7 cells. The findings demonstrated the inhibitory action of compounds 5a and 5b, with IC50 values of 42.6 ± 0.33 µM and 27.3 ± 0.28, respectively. Affinity energy and the interaction between the investigated chemicals and receptor (CDK4 ID: 2w96) were ascertained by studying molecular docking. The chemical structure-physicochemical-pharmacokinetic relationship provides useful insight for medicinal chemists to design indole-triazole derivatives with acceptable drug-like and pharmacokinetic properties.